Anatomical evidence of non-parasympathetic cardiac nitrergic nerve fibres in rat.,Journal of Anatomy

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Anatomical evidence of non-parasympathetic cardiac nitrergic nerve fibres in rat.
Journal of Anatomy ( IF 1.8 ) Pub Date : 2020-08-13 , DOI: 10.1111/joa.13291
Ieva Navickaite ₁ , Neringa Pauziene ₁ , Dainius H Pauza ₁

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Neuronal nitric oxide synthase (nNOS)‐derived nitric oxide (NO) plays a major role in the neural control of circulation and in many cardiovascular diseases. However, the exact mechanism of how NO regulates these processes is still not fully understood. This study was designed to determine the possible sources of nitrergic nerve fibres supplying the heart attempting to imply their role in the cardiac neural control. Sections of medulla oblongata, vagal nerve, its rootlets and nodose ganglia, vagal cardiac branches, Th₁‐Th₅ spinal cord segments, dorsal root ganglia of C₈‐Th₅ spinal nerves, and stellate ganglia from 28 Wistar rats were examined applying double immunohistochemical staining for nNOS combined with choline acetyltransferase (ChAT), peripherin, substance P, calcitonin gene‐related peptide, tyrosine hydroxylase or myelin basic protein. Our findings show that the most abundant population of purely nNOS‐immunoreactive (IR) neuronal somata (NS) was observed in the nodose ganglia (37.4 ± 1.3%). A high number of nitrergic NFs spread along the vagal nerve and entered its cardiac branches. All nitrergic neuronal somata (NS) in the nucleus ambiguus were simultaneously immunoreactive (IR) to ChAT and composed only a small subset of neurons (6%). In the dorsal nucleus of vagal nerve, biphenotypic nNOS‐IR/ChAT‐IR neurons composed 7.0 ± 1.0%, while small purely nNOS‐IR neurons were scarce. Nitrergic NS were plentifully distributed within the nuclei of solitary tract. In the examined dorsal root and stellate ganglia, a few nitrergic NS were sporadically present. The majority of sympathetic NS in the intermediolateral nucleus were simultaneously immunoreactive for nNOS and ChAT. In conclusion, an abundant population of nitrergic NS in the nodose ganglion implies that neuronal NO is involved in afferent cardiac innervation. Nevertheless, nNOS‐IR neurons identified within vagal nuclei may play a role in the transmission of preganglionic parasympathetic nerve impulses.

中文翻译：

大鼠非副交感神经心脏氮能神经纤维的解剖学证据。

神经元一氧化氮合酶 (nNOS) 衍生的一氧化氮 (NO) 在循环的神经控制和许多心血管疾病中起着重要作用。然而，NO 如何调节这些过程的确切机制仍未完全了解。本研究旨在确定为心脏供血的氮能神经纤维的可能来源，试图暗示它们在心脏神经控制中的作用。延髓、迷走神经、其根和结状神经节、迷走神经心脏分支、Th ₁ - Th ₅脊髓节段、C ₈ - Th _{5背根神经节的切片}对 28 只 Wistar 大鼠的脊髓神经和星状神经节进行双重免疫组化染色，检测 nNOS 联合胆碱乙酰转移酶 (ChAT)、外周蛋白、P 物质、降钙素基因相关肽、酪氨酸羟化酶或髓鞘碱性蛋白。我们的研究结果表明，在结状神经节 (37.4 ± 1.3%) 中观察到最丰富的纯 nNOS 免疫反应性 (IR) 神经元胞体 (NS)。大量的氮能 NFs 沿着迷走神经扩散并进入其心脏分支。疑核中的所有氮能神经元胞体 (NS) 同时对 ChAT 具有免疫反应 (IR)，并且仅由一小部分神经元 (6%) 组成。在迷走神经背核中，双表型 nNOS-IR/ChAT-IR 神经元占 7.0 ± 1.0%，而小的纯 nNOS-IR 神经元很少。Nitrergic NS 大量分布在孤束的细胞核内。在检查的背根和星状神经节中，零星地存在一些氮能 NS。中间外侧核中的大多数交感神经 NS 同时对 nNOS 和 ChAT 具有免疫反应性。总之，节点神经节中大量的氮能 NS 意味着神经元 NO 参与传入心脏神经支配。然而，在迷走神经核内发现的 nNOS-IR 神经元可能在节前副交感神经冲动的传递中发挥作用。总之，节点神经节中大量的氮能 NS 意味着神经元 NO 参与传入心脏神经支配。然而，在迷走神经核内发现的 nNOS-IR 神经元可能在节前副交感神经冲动的传递中发挥作用。总之，节点神经节中大量的氮能 NS 意味着神经元 NO 参与传入心脏神经支配。然而，在迷走神经核内发现的 nNOS-IR 神经元可能在节前副交感神经冲动的传递中发挥作用。

更新日期：2020-08-13

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