Background: Natural Phytoestrogens present in plants are effective hormonal replacement therapy. They are converted to estrogenic substances in the gastrointestinal tract, which is considered as the natural alternative to estrogen substitute treatment for postmenopausal women.

Aims and Objective: Salvia officinalis, a herb traditionally used to ameliorate postmenopausal complications, can provide a safe alternative to synthetic pharmaceuticals for the treatment of menopause. Therefore, it is conceivable to detect the possible estrogenic effect of Salvia Officinalis extract as an estrogen replacement therapy in female mice.

Methods: Phytochemical, pharmacological, and immune histopathological techniques are adopted in this study. HPLC is used for the identification of extracted constituents of sage herb. The uterotrophic activity of the extract was determined in immature female mice. Moreover, the mean thickness and luminal epithelium and the photomicrographs of the luminal epithelium of the uterus were also studied.

Results: HPLC revealed that quercetin is the major extracted constituent (28.6%) of the total components. Saliva officinalis extract produced a significant increase in the uterine dry weight of equal potency to estrogen. The uterus exhibited a significant increase in luminal epithelial cell height (43.3 ± 6.1μm and 36.5 ± 2.5μm) for estradiol and sage extract, respectively, compared with the control group (18.2 ± 3.5μm). Furthermore, the endometrium showed the lining epithelium formed of a single layer of columnar epithelium. The stroma seemed more voluminous with dilated vasculature. Conversely, the myometrium within the uterus was not affected in any of the experimental groups.

Conclusion: The sage herbs induced proliferative changes in the uteri of treated mice, which suggest possible estrogenic properties. Saliva officinalis extract can be used as a hormonal replacement for women during menopause and could be further explored for contraceptive use.

背景：植物中存在的天然植物雌激素是有效的激素替代疗法。它们在胃肠道中转化为雌激素物质，这被认为是绝经后妇女雌激素替代治疗的天然替代品。

目的和目的：丹参是一种传统上用于改善绝经后并发症的草药，可以为治疗更年期的合成药物提供一种安全的替代品。因此，可以想象检测丹参提取物作为雌性小鼠雌激素替代疗法可能产生的雌激素作用。

方法：本研究采用植物化学、药理学和免疫组织病理学技术。HPLC用于鉴定鼠尾草的提取成分。在未成熟的雌性小鼠中测定了提取物的促子宫活性。此外，还研究了子宫的平均厚度和管腔上皮以及管腔上皮的显微照片。

结果： HPLC 显示槲皮素是总成分的主要提取成分 (28.6%)。唾液提取物显着增加了与雌激素同等效力的子宫干重。与对照组（18.2±3.5μm）相比，雌二醇和鼠尾草提取物的子宫腔上皮细胞高度分别显着增加（43.3±6.1μm和36.5±2.5μm）。此外，子宫内膜显示由单层柱状上皮形成的衬里上皮。基质似乎更大，血管扩张。相反，在任何实验组中，子宫内的子宫肌层均未受到影响。

结论：鼠尾草诱导了治疗小鼠子宫的增殖变化，这表明可能具有雌激素特性。唾液提取物可用作更年期女性的荷尔蒙替代品，并可进一步探索用于避孕用途。