Agitated Vitex agnus castus L. shoot cultures were established to analyse the content of selected pharmaceutically important flavonoids and phenolic acids. Two variants (selected from nine ones) of MS medium were prepared: A (BAP 1 mg/L; NAA 0.5 mg/L; GA3 0.25 mg/L) and B (BAP 2 mg/L; NAA 0.5 mg/L). The biomass was harvested after 1, 2, 3,4, 5 and 6 weeks. Four‐week cultures (variant A) were selected to perform the precursor feeding experiment. The L‐phenylalanine dose of 1.6 g/L appears to be the most advantageous. Compared to the control cultures, the content of the individual compounds increased in a range from 1.4 to 17.3‐fold (e.g. p‐coumaric acid – 17.3 fold; casticin – 4.8‐fold). The biomass from in vitro cultures is richer in neochlorogenic acid (16‐fold), p‐coumaric acid (5.3‐fold), rutin (2.8‐fold), caffeic acid (1.5‐fold) and cinaroside (1.5‐fold) than the leaves of its parent greenhouse‐cultivated plants. Extracts contained 30 mg/100 g DW of casticin, but after the hydrolysis its amount increased up to 200 mg/100 g DW and twice exceeded the content in the greenhouse leaves. The results indicate that V. agnus castus agitated shoot cultures might be considered as a potential biotechnological source of some pharmaceutically important compounds, especially casticin, rutin, neochlorogenic and p‐coumaric acids.

中文翻译：

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通过前体饲喂策略提高荆条芽培养物中药学上重要的多酚化合物的产量


建立搅拌的荆条芽培养物以分析选定的药学上重要的黄酮类化合物和酚酸的含量。制备了 MS 培养基的两种变体（从九个中选择）：A（BAP 1 mg/L；NAA 0.5 mg/L；GA3 0.25 mg/L）和 B（BAP 2 mg/L；NAA 0.5 mg/L）。 1、2、3、4、5和6周后收获生物量。选择四周培养物（变体 A）来进行前体喂养实验。 1.6 g/L 的 L-苯丙氨酸剂量似乎是最有利的。与对照培养物相比，各个化合物的含量增加了 1.4 至 17.3 倍（例如对香豆酸 – 17.3 倍；蓖麻素 – 4.8 倍）。体外培养物的生物量比天然培养物更富含新绿原酸（16 倍）、对香豆酸（5.3 倍）、芦丁（2.8 倍）、咖啡酸（1.5 倍）和桂香苷（1.5 倍）。其亲本温室栽培植物的叶子。提取物中含有 30 mg/100 g DW 的蓖麻素，但水解后其含量增加至 200 mg/100 g DW，两倍于温室叶片中的含量。结果表明，V. agnuscastus 搅拌芽培养物可能被认为是一些药学上重要化合物的潜在生物技术来源，特别是蓖麻碱、芦丁、新绿原酸和对香豆酸。