A guide to: generation and design of nanobodies,The FEBS Journal

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A guide to: generation and design of nanobodies
The FEBS Journal ( IF 5.5 ) Pub Date : 2020-08-11 , DOI: 10.1111/febs.15515
Serge Muyldermans _{1,

2}

Affiliation

A nanobody (Nb) is a registered trademark of Ablynx, referring to the single antigen‐binding domain of heavy chain‐only antibodies (HCAbs) that are circulating in Camelidae. Nbs are produced recombinantly in micro‐organisms and employed as research tools or for diagnostic and therapeutic applications. They were – and still are – also named single‐domain antibodies (sdAbs) or variable domain of the heavy chain of HCAbs (VHH). A variety of methods are currently in use for the fast and efficient generation of target‐specific Nbs. Such Nbs are produced at low cost and associate with high affinity to their cognate antigen. They are robust, strictly monomeric and easy to tailor into more complex entities to meet the requirements of their application. Here, we review the various sources and different strategies that have been developed to identify rapidly, target‐specific Nbs. We further discuss a variety of engineering technologies that have been explored to broaden the application range of Nbs and summarise those applications where designed Nbs might offer a marked advantage over other affinity reagents.

中文翻译：

指南：纳米抗体的产生和设计

纳米抗体（Nb）是Ablynx的注册商标，是指在骆驼科中流通的仅重链抗体（HCAb）的单个抗原结合域。Nbs是在微生物中重组产生的，并用作研究工具或用于诊断和治疗应用。它们曾经（现在仍然）也被称为单域抗体（sdAb）或HCAbs重链的可变域（VHH）。目前，有多种方法可用于快速有效地生成特定于目标的Nb。这样的Nb以低成本生产，并与其同源抗原具有高亲和力。它们功能强大，严格为单体，并且易于定制为更复杂的实体，以满足其应用程序的要求。这里，我们回顾了已开发出的各种资源和不同的策略，这些资源和策略可用于快速确定针对特定目标的铌。我们将进一步讨论已探索的各种工程技术，以扩大Nb的应用范围，并总结那些设计Nb可能比其他亲和试剂具有明显优势的应用。

更新日期：2020-08-11

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