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Malvidin induces hepatic stellate cell apoptosis via the endoplasmic reticulum stress pathway and mitochondrial pathway
Food Science & Nutrition ( IF 3.5 ) Pub Date : 2020-08-10 , DOI: 10.1002/fsn3.1810
Yanhong Ma 1, 2 , Yahui Li 1 , Hongzhi Zhang 1 , Ying Wang 1 , Caie Wu 3 , Wuyang Huang 1, 2, 4
Affiliation  

Blueberries have great beneficial effects due to high level of anthocyanins, especially malvidin. Hepatic stellate cells (HSCs) can be activated and increase excessive extracellular matrix (ECM) components, which play a central role in liver fibrogenesis. Therefore, activated HSC’s apoptosis can be induced to recover liver fibrosis. Malvidin's effects on apoptosis in rat activated hepatic stellate T6 cells (HSC‐T6) in vitro were investigated here. High concentration of malvidin was found to significantly induce apoptosis, activate caspase‐3, increase malondialdehyde, upregulate Bax, but downregulate Bcl‐2. Moreover, malvidin upregulated the protein levels of some endoplasmic reticulum stress (ERS)‐typical markers, including caspase‐12, glucose‐regulated protein 78 (GRP78), and CCAAT/enhancer‐binding protein (C/EBP) homologous protein (CHOP), suggesting that malvidin induced HSC apoptosis by the ERS apoptosis pathway as well as the mitochondrial‐dependent pathway. These findings indicated that blueberry anthocyanins, especially malvidin, could induce activated hepatic stellate cell apoptosis and might act as one kind of functional food ingredient or a novel nutraceutical beneficial for liver health.

中文翻译:


Malvidin通过内质网应激途径和线粒体途径诱导肝星状细胞凋亡



蓝莓由于花青素含量高,尤其是锦葵素,具有很大的有益作用。肝星状细胞(HSC)可以被激活并增加过量的细胞外基质(ECM)成分,这在肝纤维形成中发挥核心作用。因此,活化的HSC细胞凋亡可以诱导肝纤维化的恢复。本文研究了马尔维丁对体外大鼠激活的肝星状 T6 细胞 (HSC-T6) 细胞凋亡的影响。高浓度的锦葵素被发现显着诱导细胞凋亡,激活 caspase-3,增加丙二醛,上调 Bax,但下调 Bcl-2。此外,马尔维丁上调了一些内质网应激(ERS)典型标志物的蛋白质水平,包括半胱天冬酶-12、葡萄糖调节蛋白78(GRP78)和CCAAT/增强子结合蛋白(C/EBP)同源蛋白(CHOP) ,表明马尔维丁通过 ERS ​​凋亡途径以及线粒体依赖性途径诱导 HSC 凋亡。这些研究结果表明,蓝莓花青素,尤其是锦葵素,可以诱导活化的肝星状细胞凋亡,可能作为一种功能性食品成分或有益于肝脏健康的新型营养保健品。
更新日期:2020-09-20
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