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Geobacter Autogenically Secretes Fulvic Acid to Facilitate the Dissimilated Iron Reduction and Vivianite Recovery.
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2020-08-06 , DOI: 10.1021/acs.est.0c01404 Shu Wang 1 , Yu Wu 1 , Jingkun An 1 , Danhui Liang 1 , Lili Tian 2 , Lean Zhou 2 , Xin Wang 2 , Nan Li 1
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2020-08-06 , DOI: 10.1021/acs.est.0c01404 Shu Wang 1 , Yu Wu 1 , Jingkun An 1 , Danhui Liang 1 , Lili Tian 2 , Lean Zhou 2 , Xin Wang 2 , Nan Li 1
Affiliation
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Biosynthetic organic matters, such as humus, play important roles in iron and phosphorus cycling in soil and aquatic systems. As an important member of humus, fulvic acid (FA) is ubiquitous in different environmental media, such as water, soil, and sediments. In this study, we fabricated the network among phosphate supply, metabolism pathway of FA, iron reduction, and vivianite recovery at the batch scale. Both the vivianite recovery performance and the content of biosynthetic FA were positively related to the phosphorus dosage. The highest vivianite formation efficiency of 53% was obtained in the Fe/P = 1 batch, accompanied with the maximal iron reduction rate of 2.29 mM·day–1, which was 2.66 times higher than that of the Fe/P = 3 batch. Simultaneously, the highest content of FA was detected in extracellular polymeric substances (EPS) of the Fe/P = 1 batch. Metabolome analysis revealed that FA biosynthesis was mainly relevant to tricarboxylic acid (TCA) cycle, amino acid metabolism, and purine metabolism, with glutamate and aspartate as the precursors. Sufficient phosphate stimulated the FA biosynthesis by modulating the biosynthesis and transformation of glutamate and aspartate. After adding 10 mg L–1 FA in Fe/P = 1 batch, the maximal iron reduction rate increase by 35%, as well as 12% improvement of the vivianite formation efficiency. Transcriptome revealed that FA promotes iron reduction and vivianite recovery by upregulating the expression of metal ion binding-, flagella-, and electron transfer activity-related genes.
中文翻译：
Geobacter会自体分泌黄腐酸，以促进异化的铁还原和Vivianite回收。
腐殖质等生物合成有机物在土壤和水生系统中的铁和磷循环中起重要作用。作为腐殖质的重要成员，富里酸（FA）在水，土壤和沉积物等不同的环境介质中无处不在。在这项研究中，我们在批处理规模上构建了磷酸盐供应，FA的代谢途径，铁还原和堇青石回收之间的网络。堇青石的回收性能和生物合成FA的含量都与磷的剂量成正相关。Fe / P = 1批次中获得的Vivianite形成效率最高，达到53％，同时最大的铁还原速率为2.29 mM·day –1，这是Fe / P = 3批次的2.66倍。同时，在Fe / P = 1批次的细胞外聚合物中（EPS）检测到FA的含量最高。代谢组学分析表明，FA的生物合成主要与三羧酸（TCA）循环，氨基酸代谢和嘌呤代谢有关，其中谷氨酸和天冬氨酸为前体。足够的磷酸盐通过调节谷氨酸和天冬氨酸的生物合成和转化来刺激FA的生物合成。加入10 mg L –1之后Fe / P = 1批次中的FA时，最大的铁还原率提高了35％，并且使堇青石的形成效率提高了12％。转录组显示FA通过上调与金属离子结合，鞭毛和电子转移活性相关的基因的表达来促进铁的还原和堇青石的回收。
更新日期：2020-09-01
中文翻译：
Geobacter会自体分泌黄腐酸，以促进异化的铁还原和Vivianite回收。
腐殖质等生物合成有机物在土壤和水生系统中的铁和磷循环中起重要作用。作为腐殖质的重要成员，富里酸（FA）在水，土壤和沉积物等不同的环境介质中无处不在。在这项研究中，我们在批处理规模上构建了磷酸盐供应，FA的代谢途径，铁还原和堇青石回收之间的网络。堇青石的回收性能和生物合成FA的含量都与磷的剂量成正相关。Fe / P = 1批次中获得的Vivianite形成效率最高，达到53％，同时最大的铁还原速率为2.29 mM·day –1，这是Fe / P = 3批次的2.66倍。同时，在Fe / P = 1批次的细胞外聚合物中（EPS）检测到FA的含量最高。代谢组学分析表明，FA的生物合成主要与三羧酸（TCA）循环，氨基酸代谢和嘌呤代谢有关，其中谷氨酸和天冬氨酸为前体。足够的磷酸盐通过调节谷氨酸和天冬氨酸的生物合成和转化来刺激FA的生物合成。加入10 mg L –1之后Fe / P = 1批次中的FA时，最大的铁还原率提高了35％，并且使堇青石的形成效率提高了12％。转录组显示FA通过上调与金属离子结合，鞭毛和电子转移活性相关的基因的表达来促进铁的还原和堇青石的回收。
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