Directly profiling intact Staphylococcus aureus in water and foods via enzymatic cleavage aptasensor,Analytica Chimica Acta

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Directly profiling intact Staphylococcus aureus in water and foods via enzymatic cleavage aptasensor
Analytica Chimica Acta ( IF 5.7 ) Pub Date : 2020-10-01 , DOI: 10.1016/j.aca.2020.07.058
Yunhao Lu ₁ , Zilan Yuan ₁ , Jinrong Bai ₁ , Qi Lin ₁ , Ruijie Deng ₁ , Aimin Luo ₂ , Yuanlong Chi ₁ , Sha Deng ₁ , Qiang He ₁

Affiliation

Staphylococcus aureus (S. aureus) causes serious food-borne diseases, and tools able to directly profile intact S. aureus would greatly facilitate food safety and public health. Herein, we proposed a biosensing platform for culture-independent and separation-free profiling S. aureus, thus allow us to directly detect intact S. aureus in complex samples. The binding protection effect of aptamer-cell complex was introduced to construct the aptasensor, and it allowed to eliminate the optimization of aptamer probe sequences. The proposed aptasensor, terms enzymatic cleavage aptasensor could achieve a sensitive (a detection limit of 64 CFU/mL) and broad-concentration quantification (dynamic range 102-107 CFU/mL) of S. aureus. Furthermore, it could specifically identify intact S. aureus in complex samples, and the quantifying of S. aureus was achieved in tap water, milk and porker with high precision. Therefore, enzymatic cleavage aptasensor could be a good candidate for on-site biosensing platform of S. aureus, as well as other pathogens by replacing the aptamer sequences.

中文翻译：

通过酶切适体传感器直接分析水和食物中完整的金黄色葡萄球菌

金黄色葡萄球菌 (S. aureus) 会导致严重的食源性疾病，能够直接分析完整金黄色葡萄球菌的工具将极大地促进食品安全和公共卫生。在此，我们提出了一个用于独立培养和免分离分析金黄色葡萄球菌的生物传感平台，从而使我们能够直接检测复杂样本中完整的金黄色葡萄球菌。适体-细胞复合物的结合保护作用被引入到构建适体传感器中，它允许消除适体探针序列的优化。提议的适体传感器，术语酶促裂解适体传感器可以实现金黄色葡萄球菌的灵敏（检测限为 64 CFU/mL）和宽浓度定量（动态范围 102-107 CFU/mL）。此外，它可以特异性地鉴定复杂样品中完整的金黄色葡萄球菌，并量化金黄色葡萄球菌。金黄色葡萄球菌是在自来水、牛奶和猪肉中高精度获得的。因此，酶切适体传感器可以通过替换适体序列成为金黄色葡萄球菌以及其他病原体的现场生物传感平台的良好候选者。

更新日期：2020-10-01

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