Self-Assembled mRNA-Responsive DNA Nanosphere for Bioimaging and Cancer Therapy in Drug-Resistant Cells.,Analytical Chemistry

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Self-Assembled mRNA-Responsive DNA Nanosphere for Bioimaging and Cancer Therapy in Drug-Resistant Cells.
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-08-04 , DOI: 10.1021/acs.analchem.0c01895
Yifan Jiang ₁ , Xin Xu ₁ , Xiao Fang ₁ , Shuxian Cai ₁ , Min Wang ₁ , Chao Xing ₂ , Chunhua Lu ₁ , Huanghao Yang ₁

Affiliation

DNA assembly has provided new opportunities for the development of a novel drug delivery system (DDS) for real-time monitoring and precision treatment of cancer lesions. Herein, we propose mRNA-responsive DNA nanospheres (DNA-NS), whose self-assembly can be triggered by products of rolling circle amplification and functional hairpins and deliver anticancer drug doxorubicin (DOX) for bioimaging and cancer therapy. It has been demonstrated that DNA-NS exhibited good stability in biological environments. Hence, DNA-NS can serve as a universal platform of detections of mRNA related to various tumor cells. DNA-NS can also be applied in the mRNA-dependent DDS. For drug-resistant cells, which are widely present in actual cancer models, DNA-NS can effectively overcome the efflux action of drug-resistant cells to improve the therapeutic efficacy of DOX. In summary, this study provides a potential strategy for constructing the endogenous mRNA-responsive DDS for cancer diagnosis and chemotherapy in vivo.

中文翻译：

自组装的mRNA响应DNA纳米球，用于在耐药细胞中进行生物成像和癌症治疗。

DNA组装为开发新型药物递送系统（DDS）提供了新的机遇，该系统可实时监测和精确治疗癌症病变。在这里，我们提出了mRNA响应性DNA纳米球（DNA-NS），其自组装可以由滚环扩增和功能性发夹的产物触发，并提供抗癌药物阿霉素（DOX）用于生物成像和癌症治疗。已经证明DNA-NS在生物环境中表现出良好的稳定性。因此，DNA-NS可以作为检测与各种肿瘤细胞相关的mRNA的通用平台。DNA-NS也可以应用于依赖mRNA的DDS。对于在实际癌症模型中广泛存在的耐药细胞，DNA-NS可以有效克服耐药细胞的外排作用，从而提高DOX的疗效。

更新日期：2020-09-01

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