基于多功能和刺激响应的聚多巴胺纳米颗粒基平台，用于靶向抗菌应用,Advanced Functional Materials

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基于多功能和刺激响应的聚多巴胺纳米颗粒基平台，用于靶向抗菌应用
Advanced Functional Materials ( IF 18.5 ) Pub Date : 2020-08-05 , DOI: 10.1002/adfm.202004503
Nesha May O. Andoy ₁ , Keuna Jeon _{1,

2} , Christian Titus Kreis ₁ , Ruby May A. Sullan _{1,

2}

Affiliation

日益增长的抗菌素耐药性威胁是全球范围的危机。如果不解决，可能会导致全球医疗保健系统问题。这保证了替代疗法的作用机理与传统的基于抗生素的疗法完全不同。在这里，通过结合聚多巴胺纳米颗粒（PdNP）的固有光热能力和出色的生物相容性以及抗微生物肽（AMP）的膜靶向和裂解活性，设计出了一种多功能且具有刺激性（NIR激光激活）的抗菌平台。所得的PdNP‐AMP纳米系统可以特异性地靶向并破坏大肠杆菌外膜的机械完整性，使用原子力显微镜测量。此外，激光诱导的PdNP纳米局部加热（紧邻已经受损的细菌包膜）会引起进一步的膜损伤。这样可以提高PdNP‐AMP的激光激活细菌杀灭效率。这项工作开发的抗菌平台被证明对耐药的大肠杆菌有效。总的来说，这项工作突出了将多种协调的杀生物机制结合到一个基于纳米材料的系统中的优势和优势，以及在治疗耐药病原体方面的前景。

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Multifunctional and Stimuli‐Responsive Polydopamine Nanoparticle‐Based Platform for Targeted Antimicrobial Applications

The rising threat of antimicrobial resistance is a crisis of a global scale. If not addressed, it can lead to health care system problems worldwide. This warrants alternative therapeutic approaches whose mechanism of action starkly differs from conventional antibiotic‐based therapies. Here, a multifunctional and stimuli‐responsive (NIR laser‐activated) antimicrobial platform is engineered by combining the intrinsic photothermal capability and excellent biocompatibility of polydopamine nanoparticles (PdNPs), with the membrane targeting and lytic activities of an antimicrobial peptide (AMP). The resulting PdNP‐AMP nanosystem can specifically target and destabilize the mechanical integrity of the outer membrane of Escherichia coli, as measured using the atomic force microscope. Furthermore, the laser‐induced nano‐localized heating of PdNP—in close proximity to the already compromised bacterial envelope—induces further membrane damage. This results in a more efficient, laser‐activated, bacterial killing action of PdNP‐AMP. The antimicrobial platform developed in this work is shown to be effective against a drug‐resistant E. coli. Overall, this work highlights the advantage and strength of combining multiple and coordinated biocidal mechanisms, into one nanomaterial‐based system and its promise in treating drug‐resistant pathogens.

更新日期：2020-10-02

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