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Trapping Methylglyoxal by Myricetin and Its Metabolites in Mice.
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2020-08-03 , DOI: 10.1021/acs.jafc.0c03471 Shuwei Zhang 1 , Liubang Xiao 2 , Lishuang Lv 2 , Shengmin Sang 1
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2020-08-03 , DOI: 10.1021/acs.jafc.0c03471 Shuwei Zhang 1 , Liubang Xiao 2 , Lishuang Lv 2 , Shengmin Sang 1
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Trapping of methylglyoxal (MGO) has been determined to be one of the potential mechanisms for dietary polyphenols to prevent chronic diseases. In this study, myricetin was demonstrated to efficiently trap MGO to generate mono- and di-MGO adducts under in vitro conditions. Furthermore, the mono- and di-MGO adducts of myricetin were detected in urine and fecal samples collected from myricetin-treated mice based on LC–MS analysis. More importantly, the mono-MGO adducts of the mono- and di-methylated myricetin were also found in these mouse samples. Further dose-dependent studies demonstrated that myricetin and its methylated metabolites significantly trapped MGO in a dose-dependent manner with the 400 mg/kg dose having the highest trapping efficacy (mono-MGO-myricetin: 272.0 ± 90.9 nM in urine and 1.05 ± 0.67 μg/g in feces; mono-MGO-mono-Me-myricetin: 135.2 ± 77.6 nM in urine and 1.16 ± 0.65 μg/g in feces; and mono-MGO-di-Me-myricetin: 17.0 ± 5.9 nM in urine and 0.19 ± 0.04 μg/g in feces) compared to the 100 and 200 mg/kg doses. In conclusion, this study demonstrates for the first time the in vivo trapping efficacy of myricetin, suggesting that intake of myricetin-containing foods has the potential to scavenge MGO in vivo and to prevent MGO-induced harmful effects to human health.
中文翻译：
杨梅酮及其代谢产物诱捕甲基乙二醛
甲基乙二醛（MGO）的诱捕已被确定为膳食多酚预防慢性疾病的潜在机制之一。在这项研究中，杨梅素被证明可以有效地诱捕MGO，在体外产生单-和双-MGO加合物。条件。此外，根据LC-MS分析，在从用杨梅素处理过的小鼠收集的尿液和粪便中检测到了杨梅素的单-和二-MGO加合物。更重要的是，在这些小鼠样品中还发现了单甲基化和二甲基化的杨梅素的单MGO加合物。进一步的剂量依赖性研究表明，杨梅素及其甲基化代谢产物以剂量依赖性方式显着捕获MGO，400 mg / kg剂量具有最高的捕获效果（单MGO-杨梅素：尿液中272.0±90.9 nM，1.05±0.67粪便中微克/克;尿中单MGO-单-我-杨梅素：尿135.2±77.6 nM，粪便中1.16±0.65μg/ g;尿中单MGO-二-我-杨梅素：17.0±5.9 nM与100和200 mg / kg剂量相比，粪便中的浓度为0.19±0.04μg/ g）。总而言之，这项研究首次证明了杨梅素在体内的诱捕功效，表明摄入含有杨梅素的食物具有清除体内MGO的潜力，并能防止MGO诱导的对人体健康的有害影响。
更新日期：2020-09-02
中文翻译：
杨梅酮及其代谢产物诱捕甲基乙二醛
甲基乙二醛（MGO）的诱捕已被确定为膳食多酚预防慢性疾病的潜在机制之一。在这项研究中，杨梅素被证明可以有效地诱捕MGO，在体外产生单-和双-MGO加合物。条件。此外，根据LC-MS分析，在从用杨梅素处理过的小鼠收集的尿液和粪便中检测到了杨梅素的单-和二-MGO加合物。更重要的是，在这些小鼠样品中还发现了单甲基化和二甲基化的杨梅素的单MGO加合物。进一步的剂量依赖性研究表明，杨梅素及其甲基化代谢产物以剂量依赖性方式显着捕获MGO，400 mg / kg剂量具有最高的捕获效果（单MGO-杨梅素：尿液中272.0±90.9 nM，1.05±0.67粪便中微克/克;尿中单MGO-单-我-杨梅素：尿135.2±77.6 nM，粪便中1.16±0.65μg/ g;尿中单MGO-二-我-杨梅素：17.0±5.9 nM与100和200 mg / kg剂量相比，粪便中的浓度为0.19±0.04μg/ g）。总而言之，这项研究首次证明了杨梅素在体内的诱捕功效，表明摄入含有杨梅素的食物具有清除体内MGO的潜力，并能防止MGO诱导的对人体健康的有害影响。
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