当前位置:
X-MOL 学术
›
Anal. Chim. Acta
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
A novel method combining aptamer-Ag10NPs based microfluidic biochip with bright field imaging for detection of KPC-2-expressing bacteria
Analytica Chimica Acta ( IF 5.7 ) Pub Date : 2020-10-01 , DOI: 10.1016/j.aca.2020.07.061
Jing Chen 1 , Hui Li 1 , Hexin Xie 2 , Danke Xu 1
Analytica Chimica Acta ( IF 5.7 ) Pub Date : 2020-10-01 , DOI: 10.1016/j.aca.2020.07.061
Jing Chen 1 , Hui Li 1 , Hexin Xie 2 , Danke Xu 1
Affiliation
![]() |
The β-lactam drugs resistance poses a serious threat to human health throughout the world. Klebsiella pneumoniae carbapenemase 2 (KPC-2) is a carbapenemase that produced in bacteria can hydrolyze carbapenems, which typically considered as the antibiotics of last resort. Therefore, there is an urgent need to quickly and accurately detect whether bacteria express KPC-2. In this paper, a PDMS/glass microfluidic biochip integrated with aptamer-modified Ag10NPs nano-biosensors was developed for rapid, simple and specific pathogenic bacteria detection, more importantly, the biochip was combined with bright field imaging, then the captured bacteria could be observed and counted directly without using extra chemical labeling. KPC-2-expressing Escherichia coli (KPC-2 E.coli) was used as the target bacterium with a detected limit of 102 CFU and capture efficiency exceeded 90%. This method is remarkably specific towards KPC-2 E.coli over other non-resistant bacteria, and pathogen assay only takes ∼1 h to complete in a ready-to-use microfluidic biochip. Furthermore, the effective capture and fast counting of microfluidic biochip system demonstrates its potential for the rapid detection of antibiotic-resistant bacteria.
中文翻译:
一种结合基于适体-Ag10NPs 的微流控生物芯片与明场成像检测表达 KPC-2 细菌的新方法
β-内酰胺类药物耐药性对全世界人类健康构成严重威胁。肺炎克雷伯菌碳青霉烯酶 2 (KPC-2) 是一种在细菌中产生的碳青霉烯酶,可以水解碳青霉烯类,通常被认为是最后的抗生素。因此,迫切需要快速准确地检测细菌是否表达KPC-2。本文开发了一种集成了适配体修饰的Ag10NPs纳米生物传感器的PDMS/玻璃微流控生物芯片,用于快速、简单和特异的病原菌检测,更重要的是,将生物芯片与明场成像相结合,可以观察捕获的细菌。并直接计数,无需使用额外的化学标记。表达 KPC-2 的大肠杆菌(KPC-2 E. 大肠杆菌)作为目标细菌,检测限为 102 CFU,捕获效率超过 90%。与其他非抗性细菌相比,这种方法对 KPC-2 E.coli 具有显着的特异性,并且病原体检测在即用型微流体生物芯片中仅需约 1 小时即可完成。此外,微流控生物芯片系统的有效捕获和快速计数证明了其在快速检测抗生素耐药细菌方面的潜力。
更新日期:2020-10-01
中文翻译:

一种结合基于适体-Ag10NPs 的微流控生物芯片与明场成像检测表达 KPC-2 细菌的新方法
β-内酰胺类药物耐药性对全世界人类健康构成严重威胁。肺炎克雷伯菌碳青霉烯酶 2 (KPC-2) 是一种在细菌中产生的碳青霉烯酶,可以水解碳青霉烯类,通常被认为是最后的抗生素。因此,迫切需要快速准确地检测细菌是否表达KPC-2。本文开发了一种集成了适配体修饰的Ag10NPs纳米生物传感器的PDMS/玻璃微流控生物芯片,用于快速、简单和特异的病原菌检测,更重要的是,将生物芯片与明场成像相结合,可以观察捕获的细菌。并直接计数,无需使用额外的化学标记。表达 KPC-2 的大肠杆菌(KPC-2 E. 大肠杆菌)作为目标细菌,检测限为 102 CFU,捕获效率超过 90%。与其他非抗性细菌相比,这种方法对 KPC-2 E.coli 具有显着的特异性,并且病原体检测在即用型微流体生物芯片中仅需约 1 小时即可完成。此外,微流控生物芯片系统的有效捕获和快速计数证明了其在快速检测抗生素耐药细菌方面的潜力。