Abstract In this study, hordein-chitosan complex particles (HCPs) were prepared using anti-solvent precipitation method. The addition of chitosan led to the increase in the particle diameter and zeta-potential as well as the gradual decrease in fluorescence intensity values of HCPs, indicating chitosan absorbed onto the surface of hordein particles. Fourier transform infrared spectra of HCPs showed the significant shifts of peak position for O–H stretching vibration, amide Ⅰ and Ⅱ bands after addition of chitosan, suggesting the formation of hydrogen bonds and hydrophobic interactions between hordein and chitosan. Three-phase contact angle (θo/w) of HCPs revealed the excellent wettability (θo/w = 89.3 ± 1°) of particles when the mass ratio of hordein to chitosan was 2:1 (HCPs2:1). Oil-in-water Pickering emulsions (Caprylic/Capric Triacylglyceride as oil phase) stabilized by HCPs2:1 were further prepared. Confocal laser scanning microscope images indicated that HCPs absorbed at the oil/water interface and formed a dense steric barrier on the surface of spherical oil droplets. Higher oil fractions (50% and 60%) led to the formation of stable Pickering emulsion gels, which showed long-term storage stability. This study provides a new solution to accelerate the application of hordein-based Pickering emulsions in food industry.

中文翻译：

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大麦醇-壳聚糖复合颗粒稳定pickering乳液的制备及表征

摘要 本研究采用反溶剂沉淀法制备大麦醇溶酶-壳聚糖复合颗粒（HCPs）。壳聚糖的加入导致 HCPs 的粒径和 zeta 电位增加，荧光强度值逐渐降低，表明壳聚糖吸附在大麦醇溶蛋白颗粒表面。HCPs的傅里叶变换红外光谱显示，加入壳聚糖后，O-H伸缩振动、酰胺Ⅰ和Ⅱ带的峰位置发生显着变化，表明大麦醇溶蛋白与壳聚糖之间形成了氢键和疏水相互作用。HCPs 的三相接触角 (θo/w) 表明，当大麦醇溶蛋白与壳聚糖的质量比为 2:1 (HCPs2:1) 时，颗粒具有优异的润湿性 (θo/w = 89.3 ± 1°)。进一步制备了由 HCPs2:1 稳定的水包油 Pickering 乳液（作为油相的辛酸/癸酸甘油三酯）。共聚焦激光扫描显微镜图像表明 HCP 在油/水界面处被吸收并在球形油滴表面形成致密的空间屏障。较高的油分率（50% 和 60%）导致形成稳定的 Pickering 乳液凝胶，显示出长期储存稳定性。该研究为加速基于大麦醇溶蛋白的 Pickering 乳液在食品工业中的应用提供了新的解决方案。较高的油分率（50% 和 60%）导致形成稳定的 Pickering 乳液凝胶，显示出长期储存稳定性。该研究为加速基于大麦醇溶蛋白的 Pickering 乳液在食品工业中的应用提供了新的解决方案。较高的油分率（50% 和 60%）导致形成稳定的 Pickering 乳液凝胶，显示出长期储存稳定性。该研究为加速基于大麦醇溶蛋白的 Pickering 乳液在食品工业中的应用提供了新的解决方案。