水溶液中的聚（甲基丙烯酸2- [二甲氨基]乙酯）-聚甲基丙烯酸羟丙酯/ DNA复合物,Journal of Polymer Science

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水溶液中的聚（甲基丙烯酸2- [二甲氨基]乙酯）-聚甲基丙烯酸羟丙酯/ DNA复合物
Journal of Polymer Science ( IF 3.9 ) Pub Date : 2020-08-01 , DOI: 10.1002/pol.20200375
Theodore Sentoukas ₁ , Stergios Pispas ₁

Affiliation

本研究涉及的聚（2- [二甲基氨基]乙基甲基丙烯酸酯）的络合能力的调查- b -聚（羟丙基丙烯酸甲酯）（PDMAEMA- b -PHPMA）两亲性的pH和温敏嵌段共聚物，以及它们的季铵化的对应物QPDMAEMA- b - PHPMA，朝向水溶液中的短DNA。该PDMAEMA- b‐PHPMA两亲嵌段共聚物插入水性介质时会表现出各种自组装特性，这取决于水性介质的组成，增溶方案，酸度和温度。通过动态光散射和在固定温度范围内的水溶液中强度的测量，研究了共聚物聚集体与DNA的相互作用以及复合后纳米结构的形成，使用了两种不同的增溶方案。通过荧光光谱和ζ电位测量进行的溴化乙锭测定也用于研究DNA /共聚物多聚体的结构和性质。这种理化特性的解释提供了对新型（Q）PDMAEMA‐ b的额外理解‐PHPMA共聚物具有自组装特性，并评估其DNA络合，稳定和递送的能力。

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Poly(2‐[dimethylamino]ethyl methacrylate)‐b‐poly(hydroxypropyl methacrylate)/DNA polyplexes in aqueous solutions

This study involves the investigation of the complexation ability of poly(2‐[dimethylamino]ethyl methacrylate)‐b‐poly(hydroxypropyl methacrylate) (PDMAEMA‐b‐PHPMA) amphiphilic pH and thermoresponsive block copolymers, and their quaternized counterparts QPDMAEMA‐b‐PHPMA, toward short DNA in aqueous solutions. The PDMAEMA‐b‐PHPMA amphiphilic block copolymers present various self‐assembly characteristics when inserted into aqueous media, depending on the composition, the solubilization protocol, the acidity and the temperature of the aqueous media. Copolymer aggregates‐DNA interactions and nanostructure formation after complexation are investigated by dynamic light scattering and intensity measurements in aqueous solutions in a fixed temperature range, utilizing two different solubilization protocols for the copolymers. Ethidium bromide assays by fluorescence spectroscopy and ζ‐potential measurements were also utilized to investigate the structure and properties of the DNA/copolymer polyplexes. The interpretation of such physicochemical characterization provides extra comprehension of the novel (Q)PDMAEMA‐b‐PHPMA copolymers self‐assembly characteristics and assesses their ability for DNA complexation, stabilization, and delivery.

更新日期：2020-08-01

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