Pairing Bacteroides vulgatus LPS Structure with Its Immunomodulatory Effects on Human Cellular Models,ACS Central Science

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Pairing Bacteroides vulgatus LPS Structure with Its Immunomodulatory Effects on Human Cellular Models
ACS Central Science ( IF 12.7 ) Pub Date : 2020-07-30 , DOI: 10.1021/acscentsci.0c00791
Flaviana Di Lorenzo _{1,

2} , Molly D. Pither ₁ , Michela Martufi ₃ , Ilaria Scarinci ₃ , Joan Guzmán-Caldentey ₄ , Ewelina Łakomiec ₅ , Wojciech Jachymek ₅ , Sven C. M. Bruijns ₆ , Sonsoles Martín Santamaría ₄ , Julia-Stephanie Frick ₇ , Yvette van Kooyk ₆ , Fabrizio Chiodo ₆ , Alba Silipo _{1,

2} , Maria Lina Bernardini ₃ , Antonio Molinaro _{1,

2}

Affiliation

The gut microbiota guide the development of the host immune system by setting a systemic threshold for immune activation. Lipopolysaccharides (LPSs) from gut bacteria are able to trigger systemic and local proinflammatory and immunomodulatory responses, and this capability strongly relies on their fine structures. Up to now, only a few LPS structures from gut commensals have been elucidated; therefore, the molecular motifs that may be important for LPS–mammalian cell interactions at the gut level are still obscure. Here, we report on the full structure of the LPS isolated from one of the prominent species of the genus Bacteroides, Bacteroides vulgatus. The LPS turned out to consist of a particular chemical structure based on hypoacylated and mono-phosphorylated lipid A and with a galactofuranose-containing core oligosaccharide and an O-antigen built up of mannose and rhamnose. The evaluation of the immunological properties of this LPS on human in vitro models revealed a very interesting capability to produce anti-inflammatory cytokines and to induce a synergistic action of MD-2/TLR4- and TLR2-mediated signaling pathways.

中文翻译：

配对拟杆菌拟杆菌LPS结构及其对人体细胞模型的免疫调节作用。

肠道菌群通过设置免疫激活的系统性阈值来指导宿主免疫系统的发育。来自肠道细菌的脂多糖（LPS）能够触发全身和局部的促炎和免疫调节反应，这种能力强烈依赖于它们的精细结构。到现在为止，仅从肠道肠道中阐明了一些LPS结构。因此，在肠道水平上可能对LPS-哺乳动物细胞相互作用重要的分子基序仍然不清楚。在这里，我们报道了从拟杆菌（Bacteroides vulgatus）的一种突出物种中分离的LPS的完整结构。事实证明，LPS由基于低酰化和单酰化的特定化学结构组成-磷酸化的脂质A，具有含半乳糖呋喃糖的核心寡糖和由甘露糖和鼠李糖形成的O抗原。对这种LPS在人体外模型上的免疫学特性的评估显示出产生抗炎细胞因子并诱导MD-2 / TLR4-和TLR2介导的信号传导途径协同作用的非常有趣的能力。

更新日期：2020-09-23

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