Efficient One-Pot Synthesis of Cytidine 5'-Monophosphate Using an Extremophilic Enzyme Cascade System.,Journal of Agricultural and Food Chemistry

当前位置： X-MOL 学术 › J. Agric. Food Chem. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Efficient One-Pot Synthesis of Cytidine 5'-Monophosphate Using an Extremophilic Enzyme Cascade System.
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2020-07-29 , DOI: 10.1021/acs.jafc.0c04055
Zonglin Li ₁ , Xiao Ning ₁ , Yiran Zhao ₁ , Xiaodan Zhang ₁ , Chun Xiao ₂ , Zhimin Li _{1,

3}

Affiliation

A rapid in vitro enzymatic biosynthesis system has been developed as a biological manufacturing platform with potential industrial uses. Cytidine 5′-monophosphate (5′-CMP) is a key intermediate in the preparation of several nucleotide derivatives and is widely used in food and pharmaceutical industries. In this study, a highly efficient biosynthesis system was constructed for manufacturing 5′-CMP in vitro. Cytidine kinase (CK) was used for the biotransformation of cytidine to 5′-CMP, while polyphosphate kinase (PPK) was coupled for adenosine triphosphate regeneration. Both CK and PPK were selected from extremophiles, possessing great potential for biocatalytic synthesis. The effects of temperature, substrate concentration, and enzyme ratios were investigated to enhance the titer and yield of 5′-CMP. After optimization, 96 mM 5′-CMP was produced within 6 h, and the yield reached nearly 100%. This work highlights the ease of 5′-CMP production by an in vitro biomanufacturing platform and provides a green and efficient approach for the industrial synthesis of 5′-CMP.

中文翻译：

使用极热酶级联系统有效地一锅合成5'-单磷酸胞苷。

快速体外酶促生物合成系统已被开发为具有潜在工业用途的生物制造平台。胞苷5'-单磷酸酯（5'-CMP）是制备几种核苷酸衍生物的关键中间体，已广泛用于食品和制药行业。在这项研究中，构建了一个高效的生物合成系统，用于体外制造5'-CMP。胞苷激酶（CK）用于将胞苷生物转化为5'-CMP，而多磷酸盐激酶（PPK）则用于三磷酸腺苷的再生。CK和PPK均选自极端嗜热菌，具有巨大的生物催化合成潜力。研究了温度，底物浓度和酶比例的影响，以提高5'-CMP的滴度和产率。优化后，在6小时内产生了96 mM 5'-CMP，产率接近100％。这项工作突出了通过体外生物制造平台生产5'-CMP的简便性，并为绿色合成5'-CMP提供了绿色有效的方法。

更新日期：2020-08-26

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南