Amplified FRET Nanoflares: An Endogenous mRNA-Powered Nanomachine for Intracellular MicroRNA Imaging.,Angewandte Chemie International Edition

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Amplified FRET Nanoflares: An Endogenous mRNA-Powered Nanomachine for Intracellular MicroRNA Imaging.
Angewandte Chemie International Edition ( IF 16.1 ) Pub Date : 2020-07-28 , DOI: 10.1002/anie.202008245
Jing Li ₁ , Jiaoli Wang ₁ , Shiyuan Liu ₁ , Nuli Xie ₁ , Ke Quan ₂ , Yanjing Yang ₃ , Xiaohai Yang ₁ , Jin Huang ₁ , Kemin Wang ₁

Affiliation

It is of great value to detect biological molecules in live cells. However, probes for imaging low‐abundance targets in live cells are limited by the one‐to‐one signal‐triggered model. Here, we introduce the concept of the amplified FRET nanoflare, which employs high‐abundance endogenous mRNA as fuel strands to amplify the detection of low abundance intracellular miRNA. As far as we know, this is the first report of an endogenous mRNA‐powered nanomachine for intracellular molecular detection. We experimentally prove the mechanism of the nanomachine and demonstrate its specificity and sensitivity. The proposed amplified FRET nanoflare can act as an excellent intracellular molecular detection strategy that is promising for biological and medical applications.

中文翻译：

放大的FRET Nanoflares：用于细胞内MicroRNA成像的内源性mRNA驱动的纳米机器。

检测活细胞中的生物分子具有重要价值。但是，用于对活细胞中低丰度靶标进行成像的探针受到一对一信号触发模型的限制。在这里，我们介绍了扩增的FRET纳米光斑的概念，它使用高丰度内源性mRNA作为燃料链来放大低丰度细胞内miRNA的检测。据我们所知，这是用于细胞内分子检测的内源性mRNA驱动纳米机器的首次报道。我们通过实验证明了纳米机的机理，并证明了其特异性和敏感性。拟议的扩增FRET纳米光斑可以作为一种出色的细胞内分子检测策略，有望在生物学和医学应用中使用。

更新日期：2020-07-28

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