Abstract This paper presents the electrochemical behavior of ticlopidine at pH 2–9. Also, for the first time, ticlopidine-hemoglobin complex used as a mediator in Hemoglobin/Ticlopidine/Titanium oxide nanoparticles nanobiocomposite (Hem/Tic/TiO2Nps) for electrocatalytic oxidation and detection of ethanol as a toxic and volatile organic compound. Furthermore, TiO2Nps was prepared by the hydrolysis and condensation reactions of titanium alkoxides. The UV-absorption spectroscopy was used for characterized of the TiO2 nanoparticles and interaction of hemoglobin with ticlopidine. The electrocatalytical effects of the electrochemical biosensor were also studied by cyclic voltammetry (CV) and differential pulse voltammetry (DPV) techniques. Under optimal conditions, ticlopidine presented a dramatical electrocatalytic behavior toward oxidation of ethanol. For proposed biosensor, linear range (40–5300 μM), detection of limit (12.1 μM) and sensitivity (0.0006 μA/μM) were obtained. This electrochemical biosensor applied to detection and determination of ethanol in simulated body fluid (SBF) solution. Finally, the Hem/Tic/TiO2Nps/GC electrode showed many advantages such as simple fabrication, low detection of limit, dramatically catalytic activity and high sensitivity.

中文翻译：

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基于血红蛋白/噻氯匹定/氧化钛纳米颗粒复合修饰电极的噻氯匹定电化学行为及乙醇检测

摘要 本文介绍了噻氯匹定在 pH 2-9 下的电化学行为。此外，噻氯匹定-血红蛋白复合物首次用作血红蛋白/噻氯匹定/氧化钛纳米颗粒纳米复合材料 (Hem/Tic/TiO2Nps) 中的介质，用于电催化氧化和检测乙醇作为有毒和挥发性有机化合物。此外，TiO2Nps 是通过钛醇盐的水解和缩合反应制备的。紫外吸收光谱用于表征二氧化钛纳米颗粒和血红蛋白与噻氯匹定的相互作用。还通过循环伏安法 (CV) 和微分脉冲伏安法 (DPV) 技术研究了电化学生物传感器的电催化效应。在最佳条件下，噻氯匹定对乙醇氧化表现出显着的电催化行为。对于提议的生物传感器，获得了线性范围 (40–5300 μM)、检测限 (12.1 μM) 和灵敏度 (0.0006 μA/μM)。该电化学生物传感器用于检测和测定模拟体液（SBF）溶液中的乙醇。最后，Hem/Tic/TiO2Nps/GC电极表现出许多优点，例如制造简单、检测限低、催化活性显着和灵敏度高。