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Dumbbell Hybridization Chain Reaction Based Electrochemical Biosensor for Ultrasensitive Detection of Exosomal miRNA.
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-07-27 , DOI: 10.1021/acs.analchem.0c02654 Peng Miao 1, 2 , Yuguo Tang 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-07-27 , DOI: 10.1021/acs.analchem.0c02654 Peng Miao 1, 2 , Yuguo Tang 1
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Exosomal miRNA is an ideal source of noninvasive biomarker for the diagnosis of cancer. Sensitive and accurate analysis of exosomal miRNA plays an important role in facilitating clinical applications. Herein, we have developed a novel electrochemical method for exosomal miRNA assay coupling strand displacement amplification (SDA) and dumbbell hybridization chain reaction (DHCR). The target triggered isothermal SDA process generates a large number of single-stranded DNA products to assist the formation of the three-way junction structure on the electrode surface. In addition, dumbbell DNA fuel strands (DHP1 and DHP2) are designed for the hybridization chain reaction. This novel form of HCR produces DNA nanostructures with a tight conformation, which facilitates the enhancement of the electrochemical response. The combination of the dual signal amplification significantly improves the sensitivity for the exosomal miRNA assay, resulting in a limit of detection (LOD) as low as 7.3 aM. More importantly, the method is successfully applied in the analysis of cells and human serum samples, which demonstrates the potential utility in point-of-care testing (POCT) applications.
中文翻译：
基于哑铃杂交链反应的电化学生物传感器，用于超敏感地检测外泌体miRNA。
外泌体miRNA是用于诊断癌症的非侵入性生物标志物的理想来源。敏感和准确的外泌体miRNA分析在促进临床应用中起着重要作用。在这里，我们已经开发了一种新颖的电化学方法，用于外泌体miRNA分析偶联链置换扩增（SDA）和哑铃杂交链反应（DHCR）。目标触发的等温SDA过程会生成大量的单链DNA产物，以协助在电极表面上形成三向连接结构。此外，哑铃DNA燃料链（DHP1和DHP2）被设计用于杂交链反应。这种新型的HCR可以产生具有紧密构象的DNA纳米结构，从而有助于增强电化学反应。双重信号放大的组合显着提高了外泌体miRNA检测的灵敏度，导致检测限（LOD）低至7.3 aM。更重要的是，该方法已成功应用于细胞和人血清样品的分析，证明了在即时检验（POCT）应用中的潜在效用。
更新日期：2020-09-01
中文翻译：
基于哑铃杂交链反应的电化学生物传感器，用于超敏感地检测外泌体miRNA。
外泌体miRNA是用于诊断癌症的非侵入性生物标志物的理想来源。敏感和准确的外泌体miRNA分析在促进临床应用中起着重要作用。在这里，我们已经开发了一种新颖的电化学方法，用于外泌体miRNA分析偶联链置换扩增（SDA）和哑铃杂交链反应（DHCR）。目标触发的等温SDA过程会生成大量的单链DNA产物，以协助在电极表面上形成三向连接结构。此外，哑铃DNA燃料链（DHP1和DHP2）被设计用于杂交链反应。这种新型的HCR可以产生具有紧密构象的DNA纳米结构，从而有助于增强电化学反应。双重信号放大的组合显着提高了外泌体miRNA检测的灵敏度，导致检测限（LOD）低至7.3 aM。更重要的是，该方法已成功应用于细胞和人血清样品的分析，证明了在即时检验（POCT）应用中的潜在效用。
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