Enzyme-coupled fluorescence sensor for sensitive determination of uric acid and uricase inhibitor,Luminescence

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Enzyme-coupled fluorescence sensor for sensitive determination of uric acid and uricase inhibitor
Luminescence ( IF 3.2 ) Pub Date : 2020-07-24 , DOI: 10.1002/bio.3919
Yuan Jiao ₁ , Yunlong Xing ₂ , Kai Li ₁ , Zainan Li ₁ , Guoqing Zhao ₁

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In this study, an enzyme-coupled fluorescence sensor was developed successfully for the sensitive detection of uric acid (UA) and uricase inhibitor based on graphene quantum dots (GQDs). UA was first oxidized using uricase and produced hydrogen peroxide (H₂O₂), which could oxidize phenol to benzoquinone in the presence of horseradish peroxidase (HRP) as the catalysis. Benzoquinone is an efficient quencher and can cause fluorescence quenching of GQDs. The degree of quenching was proportional to UA concentration and was linear for the UA concentration in the ranges 0.2–14 μmol/L. The detection limit was 0.06 μmol/L for UA. In addition, the proposed sensor was further utilized for UA detection in human serum samples with satisfactory reproducibility and accuracy. The proposed strategy provided may be widely utilized to sense H₂O₂ or H₂O₂ generating processes in related biological environment.

中文翻译：

用于灵敏测定尿酸和尿酸酶抑制剂的酶偶联荧光传感器

本研究成功开发了一种基于石墨烯量子点（GQD）的酶偶联荧光传感器，用于尿酸（UA）和尿酸酶抑制剂的灵敏检测。UA首先被尿酸酶氧化并产生过氧化氢（H ₂ O ₂)，它可以在辣根过氧化物酶 (HRP) 催化下将苯酚氧化成苯醌。苯醌是一种有效的猝灭剂，可以引起 GQD 的荧光猝灭。猝灭程度与 UA 浓度成正比，并且在 0.2-14 μmol/L 范围内与 UA 浓度呈线性关系。UA 的检出限为 0.06 μmol/L。此外，所提出的传感器进一步用于人血清样品中的 UA 检测，具有令人满意的重现性和准确性。所提出的策略可广泛用于感测相关生物环境中的H ₂ O ₂或H ₂ O ₂生成过程。

更新日期：2020-07-24

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