Aqueous two-phase extraction (ATPE) was used to extract bioactive compounds from haskap leaves (Lonicera caerulea) using salt/ethanol and sugar/propanol-based systems. The systems consisted of components that are generally recognized as safe (GRAS): ammonium sulphate/ethanol, sodium dihydrogen phosphate/ethanol, glucose/1-propanol, and maltose/1-propanol. The factors investigated were extraction time (5, 62.5, 120 min), sample loading (0.1, 0.55, 1 wt%), and tie line length (TLL) (low, medium, high) to represent different aqueous two-phase system (ATPS) compositions of salt or sugar and alcohol. Multi-response optimization was conducted to maximize the yield of chlorogenic acid, flavonoids, and total phenolic content. The results show that salt/ethanol ATPE systems had higher extraction efficiency than sugar/propanol ATPE. Among these four systems, sodium dihydrogen phosphate (NaH₂PO₄)/ethanol ATPE had the maximum extraction efficiency of 93.9% for chlorogenic acid, 96.8% for flavonoids, and 97.8% for total phenolic content. The corresponding partition coefficients were 1.73 for chlorogenic acid, 3.50 for flavonoids, and 6.59 for total phenolic content. Further analysis using high-performance liquid chromatography (HPLC) revealed that the extracts from ATPE were more refined in comparison to conventional solvent extracts. Additionally, nuclear magnetic resonance (NMR) spectra showed preliminary qualitative profiling of the extracts. This work demonstrates that ATPE using GRAS components is a green alternative to conventional extraction with organic solvents for the recovery of high-value bioactive compounds from haskap leaves.

两相水萃取（ATPE）用于从Haskap叶（忍冬）中提取生物活性化合物）使用基于盐/乙醇和糖/丙醇的系统。该系统由通常被认为是安全的（GRAS）组成：硫酸铵/乙醇，磷酸二氢钠/乙醇，葡萄糖/ 1-丙醇和麦芽糖/ 1-丙醇。研究的因素是提取时间（5、62.5、120分钟），样品上样量（0.1、0.55、1 wt％）和连接线长度（TLL）（低，中，高），以代表不同的含水两相系统（盐或糖和酒精的成分）。进行了多响应优化，以使绿原酸，类黄酮和总酚含量的产率最大化。结果表明，盐/乙醇ATPE体系的萃取效率高于糖/丙醇ATPE。在这四个系统中，磷酸二氢钠（NaH ₂ PO ₄）/乙醇ATPE对绿原酸的最大提取效率为93.9％，对黄酮类化合物的最大提取效率为98.8％，总酚含量为97.8％。绿原酸的相应分配系数为1.73，黄酮类为3.50，总酚含量为6.59。使用高效液相色谱（HPLC）进行的进一步分析显示，与常规溶剂提取物相比，ATPE提取物的提纯效果更高。此外，核磁共振（NMR）光谱显示了提取物的初步定性分析。这项工作表明，使用GRAS组分的ATPE是常规提取有机溶剂的绿色替代方法，用于从Haskap叶片中回收高价值的生物活性化合物。