We synthesized various 7-naphthylated 8-aza-7-deaza-2′-deoxyadenosine derivatives 1a–d as environmentally sensitive fluorescent (ESF) nucleosides and investigated their photophysical properties. Among them, 1b and 1c showed solvatofluorochromic properties and were able to recognized thymine base by forming Watson–Crick base pair when incorporated in DNA. Using DNA probes containing 1b and 1c, the opposite thymine base in DNA duplex was discriminated by wavelength shift of the fluorescence emission.

中文翻译：

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设计和合成7-萘基-8-氮杂-7-脱氮杂-2'-脱氧腺苷作为环境敏感的荧光核苷

我们合成了各种7-萘化的8-氮杂7-脱氮杂2'-脱氧腺苷衍生物1a-d作为对环境敏感的荧光（ESF）核苷，并研究了它们的光物理性质。其中，1b和1c显示出溶剂化荧光变色特性，当掺入DNA中时可以通过形成Watson-Crick碱基对来识别胸腺嘧啶碱基。使用含有1b和1c的DNA探针，通过荧光发射的波长偏移来区分DNA双链体中相反的胸腺嘧啶碱基。