Determination of Nitrofuran Metabolites in Fish by Ultraperformance Liquid Chromatography-Photodiode Array Detection with Thermostatic Ultrasound-Assisted Derivatization.,ACS Omega

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Determination of Nitrofuran Metabolites in Fish by Ultraperformance Liquid Chromatography-Photodiode Array Detection with Thermostatic Ultrasound-Assisted Derivatization.
ACS Omega ( IF 3.7 ) Pub Date : 2020-07-23 , DOI: 10.1021/acsomega.0c02068
Kangkang Wang ₁ , Yuli Kou ₁ , Meng Wang ₂ , Xin Ma ₂ , Jide Wang ₁

Affiliation

Nitrofuran (NF) is a class of broad-spectrum antibiotics that are used illegally in animal feeding. NF and its metabolites have proven to pose potential risk to human health. To address the current analytical needs to quantify low levels of NF metabolites in animal foods, a sensitive method was developed for simultaneous detection of four NF metabolites in fish products by an ultraperformance liquid chromatography-diode array detector (UPLC-DAD). With 2-nitrobenzaldehyde (2-NBA) as the derivatizing reagent, the metabolites were hydrolyzed and derivatized under the assistance of thermostatic ultrasound. Compared with the current detection methods, the time of the derivatization reaction has been shortened from 16 to 2 h. The relative coefficient of four NF metabolite derivatives reached more than 0.998, with excellent linear relationship. The limits of detection (LODs) and limits of quantification (LOQs) of six repeated determinations reached 0.25–0.33 and 0.80–1.10 μg/kg, respectively. For all four NF metabolites, the limit of detection of the method was below the minimum required performance limit (MRPL) of 1.0 μg/kg, which makes it compatible with the EU requirements. The recoveries ranging from 89.8 to 101.9% with relative standard deviation below 6.5% were obtained for all of the NF metabolites. What’s more, this method was successfully applied for the determination of four NF metabolites in the fish products. As a promising approach, this method could also be extended for the quantitation of NF metabolites in aquaculture and poultry products.

中文翻译：

恒温超声辅助衍生化超高效液相色谱-光电二极管阵列检测法测定鱼类中的硝基呋喃代谢物。

硝基呋喃（NF）是一类在动物饲养中非法使用的广谱抗生素。事实证明，NF及其代谢物对人体健康构成潜在风险。为了满足当前定量分析动物食品中低水平NF代谢物的分析需求，开发了一种灵敏的方法，可通过超高效液相色谱-二极管阵列检测器（UPLC-DAD）同时检测鱼产品中的四种NF代谢物。以2-硝基苯甲醛（2-NBA）为衍生试剂，代谢物在恒温超声的辅助下水解和衍生。与目前的检测方法相比，衍生化反应的时间从16小时缩短为2小时。四种NF代谢物衍生物的相对系数均大于0.998，具有良好的线性关系。六次重复测定的检出限（LODs）和定量限（LOQs）分别达到0.25-0.33和0.80-1.10μg/ kg。对于所有四种NF代谢物，该方法的检出限均低于1.0μg/ kg的最低要求性能限值（MRPL），这使其符合EU要求。所有NF代谢物的回收率在89.8％至101.9％之间，相对标准偏差低于6.5％。此外，该方法已成功应用于鱼产品中四种NF代谢物的测定。作为一种有前途的方法，该方法还可以扩展到水产养殖和家禽产品中NF代谢产物的定量分析。分别。对于所有四种NF代谢物，该方法的检出限均低于1.0μg/ kg的最低要求性能限值（MRPL），这使其符合EU要求。所有NF代谢物的回收率在89.8％至101.9％之间，相对标准偏差低于6.5％。此外，该方法已成功应用于鱼产品中四种NF代谢物的测定。作为一种有前途的方法，该方法还可以扩展到水产养殖和家禽产品中NF代谢产物的定量分析。分别。对于所有四种NF代谢物，该方法的检出限均低于1.0μg/ kg的最低要求性能限值（MRPL），这使其符合EU要求。所有NF代谢物的回收率在89.8％至101.9％之间，相对标准偏差低于6.5％。此外，该方法已成功应用于鱼产品中四种NF代谢物的测定。作为一种有前途的方法，该方法还可以扩展到水产养殖和家禽产品中NF代谢产物的定量分析。所有NF代谢物均获得9％的相对标准偏差低于6.5％。此外，该方法已成功应用于鱼产品中四种NF代谢物的测定。作为一种有前途的方法，该方法还可以扩展到水产养殖和家禽产品中NF代谢产物的定量分析。所有NF代谢物均获得9％的相对标准偏差低于6.5％。此外，该方法已成功应用于鱼产品中四种NF代谢物的测定。作为一种有前途的方法，该方法还可以扩展到水产养殖和家禽产品中NF代谢产物的定量分析。

更新日期：2020-08-04

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