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Highly Specific Recognition of Guanosine Using Engineered Base-Excised Aptamers.
Chemistry - A European Journal ( IF 3.9 ) Pub Date : 2020-07-22 , DOI: 10.1002/chem.202001835 Yuqing Li 1 , Juewen Liu 1
Chemistry - A European Journal ( IF 3.9 ) Pub Date : 2020-07-22 , DOI: 10.1002/chem.202001835 Yuqing Li 1 , Juewen Liu 1
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Purines and their derivatives are highly important molecules in biology for nucleic acid synthesis, energy storage, and signaling. Although many DNA aptamers have been obtained for binding adenine derivatives such as adenosine, adenosine monophosphate, and adenosine triphosphate, success for the specific binding of guanosine has been limited. Instead of performing new aptamer selections, we report herein a base‐excision strategy to engineer existing aptamers to bind guanosine. Both a Na+‐binding aptamer and the classical adenosine aptamer have been manipulated as base‐excising scaffolds. A total of seven guanosine aptamers were designed, of which the G16‐deleted Na+ aptamer showed the highest bindng specificity and affinity for guanosine with an apparent dissociation constant of 0.78 mm. Single monophosphate difference in the target molecule was also recognizable. The generality of both the aptamer scaffold and excised site were systematically studied. Overall, this work provides a few guanosine binding aptamers by using a non‐SELEX method. It also provides deeper insights into the engineering of aptamers for molecular recognition.
中文翻译：
使用工程碱基切除的适体对鸟苷的高度特异性识别。
嘌呤及其衍生物是生物学中用于核酸合成，能量存储和信号传导的高度重要的分子。尽管已经获得了许多用于结合腺嘌呤衍生物如腺苷，单磷酸腺苷和三磷酸腺苷的DNA适体，但是鸟嘌呤特异性结合的成功受到限制。我们没有报告新的适配子选择，而是在此报告了一种碱基切除策略，可改造现有的适配子以结合鸟苷。Na +结合适体和经典的腺苷适体均已被用作碱基切除支架。总共设计了7个鸟嘌呤适体，其中G16缺失的Na +适体对鸟苷具有最高的结合特异性和亲和力，表观离解常数为0.78 m m。目标分子中单磷酸酯的差异也可以识别。系统地研究了适体支架和切除部位的一般性。总的来说，这项工作通过使用非SELEX方法提供了一些鸟苷结合适体。它还为分子识别适体的工程设计提供了更深入的见解。
更新日期：2020-07-22
中文翻译：
使用工程碱基切除的适体对鸟苷的高度特异性识别。
嘌呤及其衍生物是生物学中用于核酸合成，能量存储和信号传导的高度重要的分子。尽管已经获得了许多用于结合腺嘌呤衍生物如腺苷，单磷酸腺苷和三磷酸腺苷的DNA适体，但是鸟嘌呤特异性结合的成功受到限制。我们没有报告新的适配子选择，而是在此报告了一种碱基切除策略，可改造现有的适配子以结合鸟苷。Na +结合适体和经典的腺苷适体均已被用作碱基切除支架。总共设计了7个鸟嘌呤适体，其中G16缺失的Na +适体对鸟苷具有最高的结合特异性和亲和力，表观离解常数为0.78 m m。目标分子中单磷酸酯的差异也可以识别。系统地研究了适体支架和切除部位的一般性。总的来说，这项工作通过使用非SELEX方法提供了一些鸟苷结合适体。它还为分子识别适体的工程设计提供了更深入的见解。
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