A novel dicyanoisophorone (DCI)-based NIR fluorophore employing 2, 4-thiazolidinediones as the modification site was designed for fluorescence imaging. The fluorophore was assessed as a switchable reporter for H₂O₂ and the probe exhibited lysosomes-targeted, a large turn-on fluorescence signal at 720 nm with a large stokes shift (150 nm) and can be used in biological systems. The ability of the novel fluorophore to emit NIR fluorescence through a “turn-on” activation mechanism makes it a promising fluorophore for in vivo imaging applications. The strategy of introducing the thiazolidinediones with the easy modification site into the fluorophore has a good application prospect to expand the application of the NIR fluorophore.

设计了一种新颖的基于二氰基异佛尔酮（DCI）的NIR荧光团，将其以2，4-噻唑烷二酮为修饰位点进行荧光成像。荧光团被评估为H ₂ O ₂的可转换报告基因，并且该探针在720 nm处具有大的斯托克斯位移（150 nm），具有溶酶体靶向，大的开启荧光信号，可用于生物系统。新型荧光团通过“开启”激活机制发射NIR荧光的能力使其成为用于体内成像应用的有前途的荧光团。将具有容易修饰位点的噻唑烷二酮引入荧光团中的策略具有广阔的应用前景，可扩大NIR荧光团的应用范围。