Specific and visual detection of copper ion (Cu²⁺) and ATP was critical for environmental and biological fields. In this work, a new rhodol-based ratiometric fluorescent probe for the reversible recognition of Cu²⁺ and ATP was designed under theoretical analysis. The Cu²⁺/ATP-induced emission intensity showed linearly proportional to the Cu²⁺/ATP concentration (0.0–10.0 μM) with the low detection limit of 0.11 μM/0.08 μM. Due to its excellent spectral properties, the reversible detection of Cu²⁺ and ATP was exploited through test strips and hydrogel. More importantly, Electrospun PMMA doped with the probe was constructed as a fluorescent sensing platform for specific and visual detection of Cu²⁺ under UV lamp by the naked eyes and the fluorescent sensing platform could be efficiently regenerated and recycled for several times by the treatment with ATP solution. Furthermore, the probe was applied to visualize Cu²⁺ and ATP in living cells and determine the content of Cu²⁺ in real soil samples which demonstrated the first reported probe for Cu²⁺ with application in edaphic ecological safety assessment. We prospected that this present work could promote the potential applications of fluorescent sensing platform in multifunctional environmental and biological portable sensing devices.

铜离子（Cu ²⁺）和ATP的特异性和视觉检测对于环境和生物领域至关重要。在这项工作中，在理论分析下，设计了一种新型的基于可逆的可逆识别Cu ²⁺和ATP的基于Rhodol的比例荧光探针。Cu ²⁺ / ATP诱导的发射强度与Cu ²⁺ / ATP浓度（0.0-10.0μM）呈线性比例关系，检出限低至0.11μM/ 0.08μM。由于其出色的光谱特性，可通过试纸和水凝胶对Cu ²⁺和ATP进行可逆检测。更重要的是，掺有探针的静电纺PMMA被构建为荧光传感平台，用于特异性和视觉检测Cu ²⁺通过ATP溶液处理，可以在裸露的紫外线灯和荧光传感平台的作用下有效地再生和循环使用几次。此外，该探针用于可视化活细胞中的Cu ²⁺和ATP并确定真实土壤样品中Cu ²⁺的含量，这证明了第一个已报道的Cu ²⁺探针在土壤生态安全评估中的应用。我们希望这项工作可以促进荧光传感平台在多功能环境和生物便携式传感设备中的潜在应用。