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A DNAzyme cascade for amplified detection of intracellular miRNA.
Chemical Communications ( IF 4.3 ) Pub Date : 2020-07-17 , DOI: 10.1039/d0cc02847a Yanan Wu 1 , Jing Li , Ke Quan , Xiangxian Meng , Xiaohai Yang , Jin Huang , Kemin Wang
Chemical Communications ( IF 4.3 ) Pub Date : 2020-07-17 , DOI: 10.1039/d0cc02847a Yanan Wu 1 , Jing Li , Ke Quan , Xiangxian Meng , Xiaohai Yang , Jin Huang , Kemin Wang
Affiliation
Inspired by the natural enzyme cascade reaction, an artificial DNAzyme cascade system is developed for the amplified detection of intracellular miR-141. The results showed that the method enormously enhanced the readout of the fluorescence signal and achieved a femtomolar detection limit.
中文翻译:
DNAzyme级联,用于扩增检测细胞内miRNA。
受天然酶级联反应的启发,开发了一种人工DNA酶级联系统,用于扩增检测细胞内miR-141。结果表明,该方法极大地增强了荧光信号的读取,并达到了飞摩尔级的检测极限。
更新日期:2020-09-01
中文翻译:
DNAzyme级联,用于扩增检测细胞内miRNA。
受天然酶级联反应的启发,开发了一种人工DNA酶级联系统,用于扩增检测细胞内miR-141。结果表明,该方法极大地增强了荧光信号的读取,并达到了飞摩尔级的检测极限。