体外消化后鲜味肽对血管紧张素I转换酶（ACE）的抑制和抗氧化活性,Journal of Agricultural and Food Chemistry

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体外消化后鲜味肽对血管紧张素I转换酶（ACE）的抑制和抗氧化活性
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2020-07-14 , DOI: 10.1021/acs.jafc.0c02797
Li Hao ₁ , Xinchang Gao ₂ , Tingyi Zhou ₁ , Jinxuan Cao ₁ , Yangying Sun ₁ , Yali Dang ₁ , Daodong Pan _{1,

3}

Affiliation

鲜味肽可以帮助减少食物中的盐分，同时仍保持咸味。鲜有研究报道食用后鲜味肽的生物活性。我们研究了胃肠消化后12种鲜味肽的生物活性。三种鲜味肽消化后显示血管紧张素I转换酶（ACE）抑制和抗氧化活性。通过HPLC-MS / MS从肽的消化溶液中鉴定出六种新的肽。其中CC，CCNK和HCHT都具有ACE抑制活性（IC ₅₀值分别为9.81、9.00和114.99μM）和抗氧化活性（强1,1-二苯基-2-吡啶基-肼基（DPPH）和2,2'-叠氮基双（3-乙基苯并噻唑啉-6-磺酸）二铵盐（ABTS）清除自由基的活性）。AHSVRF具有很强的ACE抑制活性。这些肽以剂量依赖的方式增加了人脐静脉内皮细胞培养基中一氧化氮的浓度并降低了内皮素-1的含量。对受损的HepG2细胞进行的实验表明，肽CC，CCNK和HCHT通过其细胞保护作用和减少活性氧的含量而具有抗氧化活性。结果表明，鲜味肽食用后可提供许多健康益处。

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Angiotensin I-Converting Enzyme (ACE) Inhibitory and Antioxidant Activity of Umami Peptides after In Vitro Gastrointestinal Digestion.

Umami peptides can help reduce the salt content in foods while still maintaining a savory taste. Few studies have reported the bioactivity of umami peptides after consumption. We studied the bioactivities of 12 umami peptides after gastrointestinal digestion. Three umami peptides exhibited angiotensin I-converting enzyme (ACE) inhibitory and antioxidant activity after digestion. Six novel peptides were identified from digestion solutions of the peptides by HPLC-MS/MS. Among them, CC, CCNK, and HCHT had both ACE inhibitory activity (IC₅₀ values were 9.81, 9.00, and 114.99 μM, respectively) and antioxidant activity (strong 1,1-Diphenyl-2-pycryl-hydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) free radical scavenging activities). AHSVRF had strong ACE inhibitory activity. These peptides increased the nitric oxide concentration and decreased the content of endothelin-1 in a medium of human umbilical vein endothelial cells in a dose-dependent manner. Experiments with damaged HepG2 cells showed that peptides CC, CCNK, and HCHT had antioxidant activity through their cytoprotective effects and by reducing the reactive oxygen species content. The results indicated that umami peptides may provide many health benefits after consumption.

更新日期：2020-08-05

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