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Development of a Sensitive Immunochromatographic Method Using Lanthanide Fluorescent Microsphere for Rapid Serodiagnosis of COVID-19.
ACS Sensors ( IF 8.2 ) Pub Date : 2020-07-13 , DOI: 10.1021/acssensors.0c00927 Meng Feng 1 , Jun Chen 2 , Jingna Xun 2 , Ruixue Dai 3 , Wang Zhao 3 , Hongzhou Lu 2 , Jin Xu 4 , Li Chen 1 , Guodong Sui 3 , Xunjia Cheng 1
ACS Sensors ( IF 8.2 ) Pub Date : 2020-07-13 , DOI: 10.1021/acssensors.0c00927 Meng Feng 1 , Jun Chen 2 , Jingna Xun 2 , Ruixue Dai 3 , Wang Zhao 3 , Hongzhou Lu 2 , Jin Xu 4 , Li Chen 1 , Guodong Sui 3 , Xunjia Cheng 1
Affiliation
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The SARS-CoV-2 infection that caused the COVID-19 pandemic quickly spread worldwide within two months. Rapid diagnosis of the disease and isolation of patients are effective ways to prevent and control the spread of COVID-19. Therefore, a sensitive immunofluorescent assay method was developed for rapid detection of special IgM and IgG of COVID-19 in human serum within 10 min. The recombinant nucleocapsid protein of 2019 novel coronavirus was used as capture antigen. Lanthanide, Eu(III) fluorescent microsphere, was used to qualitatively/semiquantitatively determine the solid phase immunochromatographic assay. A total of 28 clinical positive and 77 negative serum or plasma samples were included in the test. Based on the analysis of serum or plasma from COVID-19 patients and healthy people, the sensitivity and specificity of the immunochromatographic assay were calculated as 98.72% and 100% (IgG), and 98.68% and 93.10% (IgM), respectively. The results demonstrated that rapid immunoassay has high sensitivity and specificity and was useful for rapid serodiagnosis of COVID-19.
中文翻译：
使用镧系元素荧光微球开发灵敏的免疫层析方法，用于快速诊断 COVID-19。
导致 COVID-19 大流行的 SARS-CoV-2 感染在两个月内迅速在全球蔓延。快速诊断疾病和隔离患者是预防和控制 COVID-19 传播的有效方法。因此，开发了一种灵敏的免疫荧光检测方法，可在10分钟内快速检测人血清中COVID-19的特殊IgM和IgG。使用2019新型冠状病毒的重组核衣壳蛋白作为捕获抗原。镧系元素、Eu(III) 荧光微球用于固相免疫色谱法的定性/半定量测定。测试共纳入28份临床阳性和77份阴性血清或血浆样本。根据对 COVID-19 患者和健康人的血清或血浆的分析，免疫层析测定的灵敏度和特异性分别为 98.72% 和 100% (IgG)、98.68% 和 93.10% (IgM)。结果表明，快速免疫分析具有较高的灵敏度和特异性，可用于COVID-19的快速血清诊断。
更新日期：2020-08-28
中文翻译：
使用镧系元素荧光微球开发灵敏的免疫层析方法，用于快速诊断 COVID-19。
导致 COVID-19 大流行的 SARS-CoV-2 感染在两个月内迅速在全球蔓延。快速诊断疾病和隔离患者是预防和控制 COVID-19 传播的有效方法。因此，开发了一种灵敏的免疫荧光检测方法，可在10分钟内快速检测人血清中COVID-19的特殊IgM和IgG。使用2019新型冠状病毒的重组核衣壳蛋白作为捕获抗原。镧系元素、Eu(III) 荧光微球用于固相免疫色谱法的定性/半定量测定。测试共纳入28份临床阳性和77份阴性血清或血浆样本。根据对 COVID-19 患者和健康人的血清或血浆的分析，免疫层析测定的灵敏度和特异性分别为 98.72% 和 100% (IgG)、98.68% 和 93.10% (IgM)。结果表明，快速免疫分析具有较高的灵敏度和特异性，可用于COVID-19的快速血清诊断。
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