3-(4-羟基苯基)丙酸甲酯通过诱导紫苏代谢变化调节植物生长和次生代谢物积累,Plant and Soil

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3-(4-羟基苯基)丙酸甲酯通过诱导紫苏代谢变化调节植物生长和次生代谢物积累
Plant and Soil ( IF 3.9 ) Pub Date : 2020-07-14 , DOI: 10.1007/s11104-020-04625-x
Jinhu Ma , Yongheng Cao , Liangliang Sun , Ying Li , Qiong Ju , Xiaohuan Yang , Jin Xu

作为根系分泌物的成分之一，3-(4-羟基苯基)丙酸甲酯 (MHPP) 不仅在土壤中作为硝化抑制剂，而且还调节植物生长和根系结构 (RSA)；然而，MHPP 介导的植物生长的生理和分子机制在很大程度上仍不清楚。在这里，我们通过使用紫苏（Perilla frutescens）（一种药用和食用植物）整合生理学、转录组和代谢组分析，研究了 MHPP 处理对生长和次生代谢物积累的影响。MHPP 减少初级根生长，但显着诱导紫苏幼苗的侧根形成。全长转录本测序结合比较转录组学分析表明，参与碳/氮代谢和次生代谢的基因的差异表达有助于MHPP介导的植物生长。对差异表达基因的研究表明，MHPP 以不同的途径调节叶和根的生长和代谢。参与苯丙烷代谢途径的基因在根和叶之间表现出相反的表达模式，从而导致次生代谢物在根和叶中的差异积累。此外，经过 MHPP 处理的根中 PER 基因的差异表达和过氧化物酶活性的升高是调节 ROS 平衡以及木质素和酚类化合物成分的原因，从而影响植物生长和次生代谢物的积累。MHPP 调节 RSA 和代谢物谱，提高紫苏植物药用成分的含量。这些结果表明，MHPP 的应用可能是药用植物栽培的一种有用策略。

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Methyl 3-(4-hydroxyphenyl) propionate modulates plant growth and secondary metabolite accumulation by inducing metabolic changes in Perilla frutescens

As one of the components of root exudates, methyl 3-(4-hydroxyphenyl) propionate (MHPP) not only functions as a nitrification inhibitor in soil but also modulates plant growth and root system architecture (RSA); however, the physiological and molecular mechanisms underlying MHPP-mediated plant growth remain largely unclear. Here, we investigated the effects of MHPP treatment on growth and secondary metabolite accumulation by integrating physiology, transcriptome and metabolome analyses using perilla (Perilla frutescens), a type of medicinal and edible plant. MHPP reduces primary root growth but markedly induces lateral root formation in perilla seedlings. The full-length transcript sequencing combined with the comparative transcriptomic analysis revealed that the differential expression of genes involved in carbon/nitrogen metabolism and secondary metabolism contributed to the MHPP-mediated plant growth. An investigation of the differentially expressed genes showed that MHPP modulates the growth and metabolism of leaves and roots in distinct pathways. The genes involved in the phenylpropanoid metabolism pathway showed opposite expression patterns between the roots and leaves, thereby resulting in the differential accumulation of secondary metabolites in the roots and leaves. Furthermore, the differential expression of PER genes and elevated peroxidase activity in the MHPP-treated roots were responsible for modulating the equilibrium of ROS and the components of lignin and phenolic compounds, thereby affecting plant growth and secondary metabolite accumulation. MHPP modulates RSA and metabolite profiles and improves the contents of medicinal ingredients in perilla plants. These results suggested that the application of MHPP may represent a useful strategy for the medicinal plant cultivation.

更新日期：2020-07-14

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