Small-Diameter Synthetic Vascular Graft Immobilized with the REDV Peptide Reduces Early-Stage Fibrin Clot Deposition and Results in Graft Patency in Rats.,Biomacromolecules

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Small-Diameter Synthetic Vascular Graft Immobilized with the REDV Peptide Reduces Early-Stage Fibrin Clot Deposition and Results in Graft Patency in Rats.
Biomacromolecules ( IF 5.5 ) Pub Date : 2020-07-10 , DOI: 10.1021/acs.biomac.0c00457
Atsushi Mahara ₁ , Minglun Li _{1,

2} , Yuichi Ohya ₂ , Tetsuji Yamaoka ₁

Affiliation

Early-stage hemocompatibility is indispensable for manufacturing tissue-engineered vascular grafts used in regenerative medicine. In this study, we report the in vivo blood response and patency of small-diameter synthetic vascular grafts modified with the Arg-Glu-Asp-Val (REDV) peptide. Vascular grafts were prepared by casting REDV-conjugated poly(depsipeptide-co-caprolactone) on a stainless-steel mandril (diameter: 1.8 mm). After implanting the grafts into the abdominal aorta of rats for 24 h, all three control grafts without the peptide and three out of the four REDV (control sequence) peptide-modified grafts showed occlusion. The luminal surfaces of these grafts were covered with thick thrombi. In contrast, all the grafts containing the REDV peptide were patent, and their luminal surfaces were covered with a thin layer of fibrin. These results indicated that the REDV peptide on the luminal surface effectively reduced early-stage fibrin clot deposition and formed the pseudo-endothelium layer in a peptide sequence-specific manner, resulting in graft patency.

中文翻译：

用REDV肽固定的小直径合成血管移植物可减少早期纤维蛋白凝块沉积并导致大鼠移植物通畅。

早期血液相容性对于制造用于再生医学的组织工程化血管移植是必不可少的。在这项研究中，我们报告了用Arg-Glu-Asp-Val（REDV）肽修饰的小直径合成血管移植物的体内血液反应和通畅性。血管移植物通过浇铸REDV共轭的聚（depsipeptide-制备共-己内酯）在不锈钢芯棒上（直径：1.8毫米）。将移植物植入大鼠腹主动脉中24小时后，所有三个没有肽的对照移植物和四个REDV（对照序列）肽修饰的移植物中的三个移植物均显示闭塞。这些移植物的腔表面被厚的血栓覆盖。相反，所有含有REDV肽的移植物都是有专利的，其管腔表面覆盖有一层薄的纤维蛋白。这些结果表明，腔表面上的REDV肽有效地减少了早期纤维蛋白凝块的沉积，并以肽序列特异性的方式形成了假内皮层，从而导致了移植的通畅。

更新日期：2020-08-10

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