Advanced oxidation processes (AOPs) have been applied to address multiple environmental concerns including antibiotic resistance genes (ARGs). ARGs have shown an increasing threat to human health, and they are either harbored by antibiotic-resistant bacteria (ARB) or free in the environment. However, the control of ARGs has been substantially limited by their low concentration and the limited knowledge about their interfacial behavior. Herein, a novel AOP catalyst, Ag/TiO₂/graphene oxide (GO), combined with a polyvinylidene fluoride (PVDF) ultrafiltration membrane was designed with a synergistic interfacial adsorption and oxidation function to inactivate ARGs with high efficiency in both model solutions and in secondary wastewater effluent, especially when the residue concentration was low. Further analysis showed that the mineralization of bases and phosphodiesters mainly caused the inactivation of ARGs. Moreover, the interfacial adsorption and oxidation processes of ARGs were studied at the molecular level. The results showed that GO was rich in sp² backbones and functional oxygen groups, which efficiently captured and enriched the ARGs via π-π interactions and hydrogen bonds. Therefore, the photogenerated active oxygen species attack the ARGs by partially overcoming the kinetic problems in this process. The Ag/TiO₂/GO catalyst was further combined with a PVDF membrane to test its potential in wastewater treatment applications. This work offers an efficient method and a corresponding material for the inactivation and mineralization of intra/extracellular ARGs. Moreover, the molecular-level understanding of ARG behaviors on a solid–liquid interface will inspire further control strategies of ARGs in the future.

高级氧化过程 (AOP) 已应用于解决多种环境问题，包括抗生素抗性基因 (ARG)。ARG 对人类健康的威胁越来越大，它们要么被抗生素耐药细菌 (ARB) 携带，要么游离在环境中。然而，ARGs 的控制因其低浓度和对其界面行为的了解而受到很大限制。在此，一种新型 AOP 催化剂 Ag/TiO ₂/氧化石墨烯 (GO) 与聚偏二氟乙烯 (PVDF) 超滤膜相结合，设计具有协同界面吸附和氧化功能，可在模型溶液和二级废水流出物中高效灭活 ARGs，尤其是当残留物浓度较低时. 进一步分析表明，碱基和磷酸二酯的矿化主要引起ARGs的失活。此外，在分子水平上研究了ARGs的界面吸附和氧化过程。结果表明GO富含sp ²主链和功能性氧基团，通过 π-π 相互作用和氢键有效捕获和富集 ARG。因此，光生活性氧通过部分克服该过程中的动力学问题来攻击 ARG。Ag/TiO ₂ /GO 催化剂进一步与 PVDF 膜结合，以测试其在废水处理应用中的潜力。这项工作为细胞内/外ARGs的失活和矿化提供了一种有效的方法和相应的材料。此外，对 ARG 在固液界面上行为的分子水平理解将激发未来 ARG 的进一步控制策略。