Glucose oxidase and Au nanocluster co-encapsulated metal–organic frameworks as a sensitive colorimetric sensor for glucose based on a cascade reaction,New Journal of Chemistry

当前位置： X-MOL 学术 › New J. Chem. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Glucose oxidase and Au nanocluster co-encapsulated metal–organic frameworks as a sensitive colorimetric sensor for glucose based on a cascade reaction
New Journal of Chemistry ( IF 2.7 ) Pub Date : 2020-07-09 , DOI: 10.1039/c9nj06339k
Jingtian Chi _{1,

2,

3,

4} , Manli Guo _{1,

2,

3,

4} , Chi Zhang _{2,

3,

4,

5} , Yuanhong Zhang _{2,

3,

4,

5} , Shiyun Ai _{2,

3,

4,

5} , Juying Hou _{2,

3,

4,

5} , Peng Wu _{1,

2,

3,

4} , Xiangyang Li _{1,

2,

3,

4}

Affiliation

In this work, glucose oxidase and Au nanoclusters co-encapsulated in zeolitic imidazolate frameworks (GOx & AuNCs@ZIF-8) were obtained by simply mixing Zn²⁺, dimethylimidazole, glucose oxidase (GOx) and Au nanoclusters (AuNCs) at room temperature. The GOx & AuNCs@ZIF-8 composite can be used as a sensitive colorimetric sensor for glucose based on the following cascade reaction: GOx converts glucose to gluconic acid and generates H₂O₂, which acts as a substrate for AuNCs oxidizing 3,3′,5,5′-tetramethylbenzidine (TMB) to form oxidized TMB (oxTMB) detectable at 652 nm. Owing to the effective cascade reaction and protective effect of the ZIF-8 shell on enzymes, the catalytic activity of GOx & AuNCs@ZIF-8 was enhanced 19.3-fold compared with the free GOx/AuNC solution, and the glucose sensor showed high selectivity and sensitivity. Furthermore, the GOx & AuNCs@ZIF-8 composite showed high long-term storage stability and stability against digesting enzymes and chelating compounds due to the shielding effect of the ZIF-8 scaffold.

中文翻译：

葡萄糖氧化酶和金纳米簇共包裹的金属有机框架，作为基于级联反应的葡萄糖敏感比色传感器

在这项工作中，通过简单地在室温下混合Zn ²⁺，二甲基咪唑，葡萄糖氧化酶（GOx）和Au纳米团簇（AuNCs），获得了共包裹在沸石咪唑酸盐骨架（GOx和AuNCs @ ZIF-8）中的葡萄糖氧化酶和Au纳米团簇。。基于以下级联反应，GOx和AuNCs @ ZIF-8复合材料可用作葡萄糖的灵敏比色传感器：GOx将葡萄糖转化为葡萄糖酸并生成H ₂ O ₂，作为AuNC氧化3,3'，5,5'-四甲基联苯胺（TMB）形成可在652 nm检测到的氧化TMB（oxTMB）的底物。由于ZIF-8壳有效的级联反应和对酶的保护作用，与游离GOx / AuNC溶液相比，GOx和AuNCs @ ZIF-8的催化活性提高了19.3倍，并且葡萄糖传感器显示出高选择性和敏感性。此外，由于ZIF-8支架的屏蔽作用，GOx＆AuNCs @ ZIF-8复合材料显示出很高的长期存储稳定性以及对消化酶和螯合化合物的稳定性。

更新日期：2020-08-11

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南