Peroxidase-like activity of Fe-N-C single-atom nanozyme based colorimetric detection of galactose,Analytica Chimica Acta

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Peroxidase-like activity of Fe-N-C single-atom nanozyme based colorimetric detection of galactose
Analytica Chimica Acta ( IF 5.7 ) Pub Date : 2020-09-01 , DOI: 10.1016/j.aca.2020.06.027
Xiaobin Zhou ₁ , Mengke Wang ₁ , Junyang Chen ₁ , Xiaolei Xie ₁ , Xingguang Su ₁

Affiliation

Single atom nanozymes are the artificial enzymes with enzyme-like activity, which have attracted a great deal attention in recent years due to their unique merits such as remarkable stability, excellent atom utilization and low cost. Herein, a convenient and sensitive colorimetric strategy was developed for the sensing of galactose based on Fe-N-C single-atom nanozyme (Fe-SAzyme). The Fe-SAzyme was prepared through "isolation-pyrolysis" method that exhibited intrinsic peroxidase mimicking activity, which can quickly catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) to produce blue-colored oxTMB in the presence of hydrogen peroxide (H2O2). Galactose can be oxidized by galactose oxidase (Gal Ox) to generate H2O2, and Fe-SAzyme can be utilized for quantitative colorimetric detection of galactose. A good linearity between absorbance and the galactose concentration in the range of 50-500 μM was obtained with a detection limit of (LOD) 10 μM. The Fe-SAzyme based colorimetric strategy offered a rapid, convenient and economic way for galactose quantification detection, which could be used as an alternative method for galactosemia diagnosis.

中文翻译：

基于Fe-NC单原子纳米酶比色检测半乳糖的过氧化物酶活性

单原子纳米酶是一种具有类酶活性的人工酶，由于其稳定性好、原子利用率高、成本低等独特的优点，近年来备受关注。在此，基于 Fe-NC 单原子纳米酶 (Fe-SAzyme) 开发了一种方便且灵敏的比色策略来检测半乳糖。Fe-SAzyme通过“分离-热解”方法制备，具有内在的过氧化物酶模拟活性，可快速催化3,3',5,5'-四甲基联苯胺（TMB）氧化产生蓝色oxTMB过氧化氢 (H2O2)。半乳糖可被半乳糖氧化酶（Gal Ox）氧化生成H2O2，Fe-SAzyme可用于半乳糖的定量比色检测。在 50-500 μM 范围内，吸光度与半乳糖浓度之间具有良好的线性关系，检测限为 (LOD) 10 μM。基于 Fe-SAzyme 的比色策略为半乳糖定量检测提供了一种快速、方便和经济的方法，可作为半乳糖血症诊断的替代方法。

更新日期：2020-09-01

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