The bioaugmentation is crucial to improve the energy-efficient process for anaerobic digestion of organic wastes at high ammonia levels. Genomic insights into the intricate microbial networks at a high ammonia level remain underexplored. The present study showed that the addition of Methanoculleus sp. DTU887 remarkably enhanced the methane production yield of organic fraction of municipal solid waste by 21% and decreased the volatile fatty acids by 10% when compared to the period before bioaugmentation. Genome-centric metagenomics reports the functional contribution of microbial members during organic waste degradation under the extremely high level of 13.5 g NH₄⁺-N/L. Specifically, metabolic reconstruction revealed that these organisms have the potential to perform fermentative and acetogenic catabolism, a process facilitated by energy conservation-related with H₂/CO₂ metabolism. Peptococcaceae spp. (DTU903, DTU900, and DTU895). and Tissierellales sp. DTU879 could degrade the organic waste hydrolysis product, i.e., glucose to acetate and H₂. Tissierellales sp. DTU879 and Syntrophaceticus sp. DTU783 could degrade the derived acetate. The H₂ scavenging Methanoculleus sp. DTU887 performs complementary metabolic reactions with Peptococcaceae spp., Tissierellales sp. and Syntrophaceticus sp., indicating syntrophic glucose and acetate degradation. This research offers the first insight that the key organisms form a syntrophy-supported food web in response to the bioaugmentation with ammonia tolerant methanogens performed in an AD system subjected to severe ammonia inhibition.

生物强化对于提高高氨水平下有机废物厌氧消化的能效过程至关重要。在高氨水平下对复杂微生物网络的基因组学见解仍未得到充分探索。本研究表明，添加了甲烷菌。与生物强化之前相比，DTU887显着提高了城市固体废物有机部分的甲烷产量，提高了21％，挥发性脂肪酸降低了10％。以基因组为中心的宏基因组学报道了在极高的13.5 g NH ₄ ⁺水平下微生物废物降解过程中微生物成员的功能贡献-N / L。具体而言，代谢重建表明这些生物具有进行发酵和产乙酸分解代谢的潜力，这一过程由与H ₂ / CO ₂代谢相关的能量节约促进。肽球菌属 （DTU903，DTU900和DTU895）。和Tissierellales sp。DTU879可以将有机废物水解产物，即葡萄糖降解为乙酸盐和H ₂。Tissierellales sp。DTU879和Syntrophaceticus SP。DTU783可以降解衍生的乙酸盐。H ₂清除甲烷菌。DTU887进行互补以与代谢反应Peptococcaceae属，Tissierellales sp。和Syntrophaceticus sp。，表明同养葡萄糖和乙酸盐降解。这项研究提供了第一个见识，即关键生物形成了由营养耐受性强的AD系统进行的耐氨性产甲烷菌对生物的强化作用，从而形成了由营养支持的食物网。