基于立体复合物PDLA / PLLA和明胶的混合水凝胶用于骨再生,Journal of Applied Polymer Science

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基于立体复合物PDLA / PLLA和明胶的混合水凝胶用于骨再生
Journal of Applied Polymer Science ( IF 2.7 ) Pub Date : 2020-07-01 , DOI: 10.1002/app.49571
Hecheng Wang ₁ , Enhui Wang ₁ , Yubin Huang ₂ , Xiaoyuan Li ₂

Affiliation

天然骨是致密壳皮质骨和多孔核海绵状海绵的完美结合。受这种梯度结构的启发，我们制备了双层水凝胶，其中立体复合物PDLA-PEG-PDLA和PLLA-PEG-PLLA为内部多孔核，明胶/纳米羟基磷灰石为外部增强壳。明胶与天然交联剂Genipin的交联使外壳可用于早期支撑，内部物理水凝胶促进了新骨的形成。¹证实了PDLA-PEG-PDLA（或PLLA-PEG-PLLA）的成功合成1 H NMR（代表核磁共振）光谱。复合支架的程序通过扫描电子显微镜和傅里叶变换红外光谱进行监测。小鼠前成骨细胞MC3T3-E1细胞的体外测试发现，获得的杂化水凝胶可以改善细胞粘附，增殖，钙沉积并上调成骨相关基因的表达。进一步的大鼠颅骨缺损的体内实验也证实了准备好的支架能够促进新骨形成。上述数据表明，这种核壳水凝胶有助于营养物质的运输，骨桥和骨传导性。但是如何控制水凝胶的降解过程并与新的骨骼形成匹配仍然是一个挑战。

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Hybrid hydrogel based on stereocomplex PDLA/PLLA and gelatin for bone regeneration

Natural bone is a perfect combination of dense shell cortical bone and porous core spongiosa. Inspired by this gradient structure, we prepared a double‐layered hydrogel with stereocomplex PDLA‐PEG‐PDLA and PLLA‐PEG‐PLLA as the inner porous core and gelatin/nano hydroxyapitite as the outer strengthen shell. Crosslinking of gelatin with natural crosslinker genipin made the shell available for early supporting and the inner physical hydrogel facilitated new bone formation. Successful synthesis of PDLA‐PEG‐PDLA (or PLLA‐PEG‐PLLA) was confirmed by ¹H NMR (stands for nuclear magnetic resonance) spectra. The procedure of composite scaffold was monitored with scanning electron microscopy and fourier‐transform infrared spectroscopy. In vitro test with mouse preosteoblast MC3T3‐E1 cells found that the obtained hybrid hydrogels could improve cell adhesion, proliferation, calcium deposition, and upregulate osteogenesis‐related gene expression. Further in vivo experiment on rat calvarial defects also confirmed the capacity of the as‐prepared scaffold to accelerate new bone formation. The above data suggested that this core‐shell hydrogel helpful for nutrient transportation, bony bridging, and osteoconductivity. But how to control the degradation process of the hydrogel and match with new bone formation still remained challenging.

更新日期：2020-07-01

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