To improve in vitro photostability and enhance insecticidal activity, a novel esterase/glutathione (GSH) responsive photoactivated nano-pesticide delivery system was synthesized by conjugation of photoactivated pesticide phloxine B(PB) to sodium alginate (SA) via esterase/GSH sensitive phenolic ester bond followed by ultrasonic dispersion. The system was stable in PBS (pH 7.4) and could protect effectively the conjugated PB from in vitro photodegradation because of aggregation-caused quenching effect, whose maximum photodegradation rate did not exceed 10 % after 270 min illumination. However, upon exposure to esterase-6 or GSH stimulus, high photoactivity was observed due to the destruction of the system and accompanied by PB release. The combined stimulation could trigger more PB release than any single stimulus and thus resulting in a higher photoactivity. Compared with free PB, The system showed a higher phototoxicity on Sf9 insect cells and the in vitro light exposure had little influence on the phototoxicity.

为了提高体外的光稳定性和增强杀虫活性，通过酯酶/ GSH敏感的酚酯将光活化农药福禄星B（PB）与藻酸钠（SA）缀合，合成了一种新型的酯酶/谷胱甘肽（GSH）响应型光活化纳米农药递送系统。结合，然后进行超声分散。该体系在PBS（pH 7.4）中稳定，并且由于聚集引起的猝灭作用，可以有效地保护缀合的PB免受体外光降解，其在光照270分钟后的最大光降解率不超过10％。但是，暴露于酯酶-6或GSH刺激后，由于破坏了系统并伴随着PB释放，观察到了高光活性。组合刺激可以触发比任何单个刺激更多的PB释放，从而导致更高的光活性。与游离PB相比，该系统对Sf9昆虫细胞表现出更高的光毒性，并且体外光暴露对其光毒性影响很小。