<br>成纤维细胞的短暂支持足以驱动工程组织中的功能性血管化,Advanced Functional Materials

当前位置： X-MOL 学术 › Adv. Funct. Mater. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

成纤维细胞的短暂支持足以驱动工程组织中的功能性血管化
Advanced Functional Materials ( IF 18.5 ) Pub Date : 2020-06-25 , DOI: 10.1002/adfm.202003777
H-H Greco Song ₁ , Alex Lammers ₂ , Subramanian Sundaram ₂ , Logan Rubio ₂ , Amanda X Chen ₃ , Linqing Li ₂ , Jeroen Eyckmans ₂ , Sangeeta N Bhatia ₁ , Christopher S Chen ₂

Affiliation

毛细血管的形成是天然和工程器官的关键要求，可以通过内皮细胞与成纤维细胞共培养在体外诱导。然而，这些成纤维细胞是否仅在内皮细胞的初始形态发生中需要，还是自始至终都需要，目前尚不清楚，并且在组装后去除这些基质细胞的能力可用于临床转化。在这项研究中，引入了一种称为CAMEO（用于工程器官发生的多细胞组织中的受控细胞凋亡）的技术，根据需要选择性地消融成纤维细胞，并利用它来探讨成纤维细胞在血管形态发生中的可有可无。研究表明，成纤维细胞的存在仅在内皮细胞形态发生的最初几天内是必要的，之后可以将它们消融，而不会显着影响已发育的脉管系统的结构和功能特征。此外，还证明了使用 CAMEO 使含有原代人肝细胞的构建体血管化，从而改善组织功能。总之，这项研究表明，成纤维细胞的短暂、初始支持足以驱动工程组织中的血管形态发生，这种通过消除进行工程的策略可能为在工程器官中实现所需功能和细胞组成提供一种新的通用方法。

"点击查看英文标题和摘要"

Transient Support from Fibroblasts is Sufficient to Drive Functional Vascularization in Engineered Tissues

Formation of capillary blood vasculature is a critical requirement for native as well as engineered organs and can be induced in vitro by coculturing endothelial cells with fibroblasts. However, whether these fibroblasts are required only in the initial morphogenesis of endothelial cells or needed throughout is unknown, and the ability to remove these stromal cells after assembly can be useful for clinical translation. In this study, a technique termed CAMEO (Controlled Apoptosis in Multicellular Tissues for Engineered Organogenesis) is introduced, whereby fibroblasts are selectively ablated on demand, and it is utilized to probe the dispensability of fibroblasts in vascular morphogenesis. The presence of fibroblasts is shown to be necessary only during the first few days of endothelial cell morphogenesis, after which they can be ablated without significantly affecting the structural and functional features of the developed vasculature. Furthermore, the use of CAMEO to vascularize a construct containing primary human hepatocytes that improved tissue function is demonstrated. In conclusion, this study suggests that transient, initial support from fibroblasts is sufficient to drive vascular morphogenesis in engineered tissues, and this strategy of engineering‐via‐elimination may provide a new general approach for achieving desired functions and cell compositions in engineered organs.

更新日期：2020-06-25

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南