Rare ginsenoside CK was recognized as a popular functional food because of superior pharmacological activity, but it is restricted by further applications by the difficulty in preparation. In this study, deep eutectic solvent (DES)-based aqueous two-phase system (ATPS) was established to transform and extract ginsenoside CK in situ for the first time. The phase formation conditions for preparing ATPS using choline chloride-based DES were studied, and the optimal conditions for extractive bioconversion were explored using conventional single-factor experiments. The conditions for ATPS establishment were as follows: 31.9% (w/w) DES (ChCl-ethylene glycol)/24.5% (w/w) K₂HPO₄, 55 °C, pH 5.0. Under the optimal conditions, 75.79% product and 61.14% β-glucosidase were recovered from the top and bottom phase, respectively. In addition, DES and β-glucosidase can be recovered and recycled again for the next extractive bioconversion of CK. These results indicated that this green and efficient method exhibited considerable value in integrated production and extraction processes, and demonstrated the potential for obtaining highly recycled functional foods and similar products.

稀有的人参皂苷CK由于具有优异的药理活性而被公认为是一种流行的功能食品，但由于制备困难而受到进一步应用的限制。在这项研究中，建立了基于低共熔溶剂（DES）的水两相系统（ATPS），以首次就地转化和提取人参皂苷CK 。研究了基于氯化胆碱的DES制备ATPS的相形成条件，并利用常规单因素实验探索了最佳的萃取生物转化条件。建立ATPS的条件如下：31.9％（w / w）DES（ChCl-乙二醇）/24.5%（w / w）K ₂ HPO ₄，55℃，pH 5.0。在最佳条件下，从上层和下层相中分别回收了75.79％的产物和61.14％的β-葡萄糖苷酶。另外，可以回收DES和β-葡萄糖苷酶并再次循环用于CK的下一次提取生物转化。这些结果表明，这种绿色高效的方法在集成的生产和提取过程中显示出可观的价值，并证明了获得高度回收的功能食品和类似产品的潜力。