绘制自花冬黑麦群体的茎锈病（Puccinia graminis f. sp. secalis）抗性。,Frontiers in Plant Science

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绘制自花冬黑麦群体的茎锈病（Puccinia graminis f. sp. secalis）抗性。
Frontiers in Plant Science ( IF 4.1 ) Pub Date : 2020-05-26 , DOI: 10.3389/fpls.2020.00667
Paul Gruner ₁ , Anne-Kristin Schmitt ₂ , Kerstin Flath ₂ , Brigitta Schmiedchen ₃ , Jakob Eifler ₃ , Andres Gordillo ₃ , Malthe Schmidt ₃ , Viktor Korzun _{4,

5} , Franz-Joachim Fromme ₆ , Dörthe Siekmann ₆ , Anna Tratwal ₇ , Jakub Danielewicz ₇ , Marek Korbas ₇ , Karol Marciniak ₈ , Renata Krysztofik ₈ , Małgorzata Niewińska ₈ , Silvia Koch ₁ , Hans-Peter Piepho ₉ , Thomas Miedaner ₁

Affiliation

由 Puccinia graminis f. 引起的黑麦茎锈病。sp。欧洲所有黑麦种植区都可以找到 secalis。当夏季温暖干燥时，该病害会导致大面积严重减产。迄今为止，欧洲很少进行引发抗性育种的研究。据我们所知，目前在德国注册的所有品种都受到影响。在这项研究中，研究了三个自交系双亲群体和一个为绘制抗性而开发的测交群体。两年多的时间里，每个人群测试了 68-70 个基因型，每个基因型在三个地点进行了测试。将表型数据与定制 10k Infinium iSelect 单核苷酸多态性 (SNP) 阵列的基因分型结果相结合，我们鉴定了定量遗传的成年植物抗性和单基因全阶段抗性。可以在两个独立发育的群体中鉴定出位于7R染色体远端的单一抗性基因，暂时命名为Pgs1。它很可能与核苷酸结合富含亮氨酸重复序列 (NB-LRR) 抗性基因同源物密切相关。设计了一种用于竞争性等位基因特异性聚合酶链反应 (KASP) 基因分型测定的标记，可以分别解释两个群体中 73% 和 97% 的遗传变异。对自然发生的黑麦叶锈病（由隐秘柄锈菌 ROEBERGE 引起）的进一步研究揭示了 7R 染色体上的基因复合体。Pgs1基因和进一步鉴定的数量性状位点(QTL)具有很大的潜力用于育种抗茎锈病黑麦。

"点击查看英文标题和摘要"

Mapping Stem Rust (Puccinia graminis f. sp. secalis) Resistance in Self-Fertile Winter Rye Populations.

Rye stem rust caused by Puccinia graminis f. sp. secalis can be found in all European rye growing regions. When the summers are warm and dry, the disease can cause severe yield losses over large areas. To date only little research was done in Europe to trigger resistance breeding. To our knowledge, all varieties currently registered in Germany are susceptible. In this study, three biparental populations of inbred lines and one testcross population developed for mapping resistance were investigated. Over 2 years, 68-70 genotypes per population were tested, each in three locations. Combining the phenotypic data with genotyping results of a custom 10k Infinium iSelect single nucleotide polymorphism (SNP) array, we identified both quantitatively inherited adult plant resistance and monogenic all-stage resistance. A single resistance gene, tentatively named Pgs1, located at the distal end of chromosome 7R, could be identified in two independently developed populations. With high probability, it is closely linked to a nucleotide-binding leucine-rich repeat (NB-LRR) resistance gene homolog. A marker for a competitive allele-specific polymerase chain reaction (KASP) genotyping assay was designed that could explain 73 and 97% of the genetic variance in each of both populations, respectively. Additional investigation of naturally occurring rye leaf rust (caused by Puccinia recondita ROEBERGE) revealed a gene complex on chromosome 7R. The gene Pgs1 and further identified quantitative trait loci (QTL) have high potential to be used for breeding stem rust resistant rye.

更新日期：2020-05-26

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