Introduction

Escherichia coli

strains possess two survival strategies to endure lethal antibiotic exposure including antibiotic resistance and persistence, in which persistence can contribute to the emergence of antibiotic resistance and increasing the risk of multidrug resistance. Using high-throughput proteomics for the comprehensive understanding of mechanisms of antibiotic resistance and persistence is an effective strategy for development of target-based anti-bacterial therapies.

Areas covered

In this review, we summarize a comprehensive proteomic perspective of antibiotic resistance and persistence in E. coli, and overview of anti-antibiotic resistance and anti-persister molecules and strategies for the development of potential therapies.

Expert opinion

Proteomics allows us to globally identify the critical proteins and pathways involved in antibiotic resistance and persistence. Advancements in methodologies of proteomics and multi-omic strategies are required to overcome the limitations of proteomics and better understand mechanisms of antibiotic resistance and persistence in E. coli, and to open the possibility for identification of new targets for alternative strategies in therapeutics.

中文翻译：

---

大肠杆菌中抗生素耐药性和持久性的蛋白质组学研究——医学研究的进展和潜力。

简介

大肠杆菌

菌株具有两种生存策略来承受致命的抗生素暴露，包括抗生素耐药性和持续性，其中持续性可能导致抗生素耐药性的出现并增加多药耐药性的风险。使用高通量蛋白质组学全面了解抗生素耐药性和持久性机制是开发靶向抗菌疗法的有效策略。

覆盖区域

在这次审查中，我们总结了抗生素耐药性和持久性的综合蛋白质组学角度E. 大肠杆菌和抗耐药性和抗留存分子和战略的潜在疗法的发展概况。

专家意见

蛋白质组学使我们能够在全球范围内确定与抗生素耐药性和持久性有关的关键蛋白质和途径。在蛋白组学和多组学策略方法的进步都需要克服蛋白质组学的限制，更好地了解耐药性和持续性的机制E. 大肠杆菌，并打开发现新的目标在治疗替代战略的可能性。