Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Vertical Flow Assay for Inflammatory Biomarkers Based on Nanofluidic Channel Array and SERS Nanotags.
Small ( IF 13.0 ) Pub Date : 2020-06-22 , DOI: 10.1002/smll.202002801
Ruipeng Chen 1, 2, 3 , Xin Du 1 , Yujun Cui 1, 2, 3 , Xieyuan Zhang 1, 2, 3 , Qinyu Ge 1, 2 , Jian Dong 1, 2, 3 , Xiangwei Zhao 1, 2, 3
Small ( IF 13.0 ) Pub Date : 2020-06-22 , DOI: 10.1002/smll.202002801
Ruipeng Chen 1, 2, 3 , Xin Du 1 , Yujun Cui 1, 2, 3 , Xieyuan Zhang 1, 2, 3 , Qinyu Ge 1, 2 , Jian Dong 1, 2, 3 , Xiangwei Zhao 1, 2, 3
Affiliation
|
There is a great demand for the development of detection assays for inflammation infection diagnosis with high throughput and ultrasensitivity. Herein, a vertical flow assay system with functionalized nanoporous anodic aluminum oxide (AAO) as sensing membrane, and encoded core–shell surface enhanced Raman scattering (SERS) nanotags as labels for multiple inflammatory biomarkers detection is presented. A 2 × 2 test array on the porous AAO is developed and modified with multiple capture antibodies to capture inflammatory biomarkers from samples. Due to the high surface area to volume ratio of the AAO membrane, and its influence on plasmonic coupling, the electromagnetic field of the encoded core–shell SERS nanotags is enhanced. Detection limits of 53.4, 4.72, 48.3, and 7.53 fg mL−1 are realized for C reactive protein, interleukin‐6, serum amyloid A, and procalcitonin, respectively, with a linear dynamic range spanning at least five orders of magnitude. In addition, the proposed method also shows acceptable accuracy and repeatability for the detection of clinical samples. Therefore, this approach is expected to be a powerful point of care testing tool for disease diagnosis in facility limited areas.
中文翻译：
基于纳米流体通道阵列和SERS纳米标签的炎性生物标记物的垂直流分析。
迫切需要开发用于以高通量和超敏度进行炎症感染诊断的检测测定法。本文介绍了一种垂直流分析系统，该系统具有功能化的纳米多孔阳极氧化铝（AAO）作为传感膜，并具有编码的核-壳表面增强拉曼散射（SERS）纳米标签作为多种炎症生物标志物检测的标签。开发并使用多种捕获抗体修饰多孔AAO上的2×2测试阵列，以捕获样品中的炎症生物标志物。由于AAO膜的高表面积体积比及其对等离子体耦合的影响，编码核-壳SERS纳米标签的电磁场得以增强。检测限为53.4、4.72、48.3和7.53 fg mL -1C反应蛋白，白细胞介素-6，血清淀粉样蛋白A和降钙素分别实现了线性动态范围，至少跨越了五个数量级。另外，所提出的方法对于临床样品的检测也显示出可接受的准确性和可重复性。因此，该方法有望成为设施受限区域中疾病诊断的强有力的护理测试工具。
更新日期：2020-08-14
中文翻译：
基于纳米流体通道阵列和SERS纳米标签的炎性生物标记物的垂直流分析。
迫切需要开发用于以高通量和超敏度进行炎症感染诊断的检测测定法。本文介绍了一种垂直流分析系统，该系统具有功能化的纳米多孔阳极氧化铝（AAO）作为传感膜，并具有编码的核-壳表面增强拉曼散射（SERS）纳米标签作为多种炎症生物标志物检测的标签。开发并使用多种捕获抗体修饰多孔AAO上的2×2测试阵列，以捕获样品中的炎症生物标志物。由于AAO膜的高表面积体积比及其对等离子体耦合的影响，编码核-壳SERS纳米标签的电磁场得以增强。检测限为53.4、4.72、48.3和7.53 fg mL -1C反应蛋白，白细胞介素-6，血清淀粉样蛋白A和降钙素分别实现了线性动态范围，至少跨越了五个数量级。另外，所提出的方法对于临床样品的检测也显示出可接受的准确性和可重复性。因此，该方法有望成为设施受限区域中疾病诊断的强有力的护理测试工具。
京公网安备 11010802027423号