Improving the nitrogen removal capacity of stormwater runoff has been the main research direction of bioretention systems in recent years. Heterotrophic denitrification in traditional bioretention systems was often used, but insufficient carbon sources in the runoff limit it. In this study, two bioretention systems using natural pyrite and zeolite as substrates were constructed to explore the feasibility of pyrite-based autotrophic denitrification in bioretention for nitrogen removal. During eight months of operation at natural conditions, the results showed that pyrite had no negative effects on the removal of NH₄⁺—N, but enhanced denitrification in the bioretention system with no or low carbon source in the influent. Pyrite based bioretention system (PBS) can adapt to low temperature and irregular wet and dry alternation. The average NH₄⁺—N, TN, and TP removal efficiency of PBS during eight months were 87.6 %, 89.3 %, and 81.6 %. The average NH₄⁺—N, TN, and TP removal efficiency of zeolite-based bioretention system (ZBS) were 98.7 %, 47.1 %, and 47.5 %. By-products ($S{O}_4^{2-}$ and Total iron) of PBS were all at an acceptable level. The dominant genera in PBS were Denitratisoma (3.6 %), Ellin6067 (2.7 %), Thiobacillus (2.6 %), Thauera (2.1 %), CL500−29 marine group (1.8 %), Sulfuritalea (1.7 %), Subgroup 10 (1.4 %). Genera Thiobacillus, Thauera, and Sulfuritalea were highly related to autotrophic denitrification. Moreover, genera Denitratisoma was highly related to heterotrophic denitrification. This study shows that pyrite could be a promising eco-friendly substrate in the bioretention system for nutrient removal.

近年来，提高雨水径流除氮能力一直是生物截留系统的主要研究方向。传统的生物截留系统经常使用异养反硝化，但径流中碳源不足限制了它。在这项研究中，构建了两个使用天然黄铁矿和沸石作为底物的生物保留系统，以探索基于黄铁矿的自养反硝化技术在生物保留中去除氮的可行性。在自然条件下运行八个月后，结果表明黄铁矿对NH ₄ ⁺的去除没有负面影响。-N，但进水中没有碳源或碳源低的生物保留系统中的反硝化作用增强。基于黄铁矿的生物保留系统（PBS）可以适应低温以及不规则的干湿交替。八个月内，PBS的平均NH ₄ ⁺ -N，TN和TP去除效率分别为87.6％，89.3％和81.6％。沸石基生物滞留系统（ZBS）的平均NH ₄ ⁺ -N，TN和TP去除效率分别为98.7％，47.1％和47.5％。副产品（$\mathrm{小号}{Ø}_4^{2--}$PBS的总铁和总铁都在可接受的水平。在PBS中占主导地位的属有Denitratisoma（3.6％），Ellin6067（2.7％），硫杆菌属（2.6％），Thauera（2.1％），CL500-29海洋组（1.8％），Sulfuritalea（1.7％），亚组10（1.4 ％）。属硫，Thauera和Sulfuritalea是高度相关的自养反硝化。此外，Denistratisoma属与异养反硝化高度相关。这项研究表明，黄铁矿可能是生物保留系统中用于去除养分的有前途的生态友好基质。