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Surface-Plasmonic-Field-Induced Photoredox Catalysis and Mediated Electron Transfer for Washing-Free DNA Detection.
Angewandte Chemie International Edition ( IF 16.1 ) Pub Date : 2020-06-17 , DOI: 10.1002/anie.202007318
Jihyeon Kim 1 , Jongkyoon Park 2 , Seonhwa Park 1 , Jeongwook Seo 1 , Jeongwook Kwon 1 , Hyunsoo Lee 2 , Seungchul Kim 2, 3 , Haesik Yang 1
Angewandte Chemie International Edition ( IF 16.1 ) Pub Date : 2020-06-17 , DOI: 10.1002/anie.202007318
Jihyeon Kim 1 , Jongkyoon Park 2 , Seonhwa Park 1 , Jeongwook Seo 1 , Jeongwook Kwon 1 , Hyunsoo Lee 2 , Seungchul Kim 2, 3 , Haesik Yang 1
Affiliation
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Distance‐dependent electromagnetic radiation and electron transfer have been commonly employed in washing‐free fluorescence and electrochemical bioassays, respectively. In this study, we combined the two distance‐dependent phenomena for sensitive washing‐free DNA detection. A distance‐dependent surface plasmonic field induces rapid photoredox catalysis of surface‐bound catalytic labels, and distance‐dependent mediated electron transfer allows for rapid electron transfer from the surface‐bound labels to the electrode. An optimal system consists of a chemically reversible acceptor (Ru(NH3)63+), a chemically reversible photoredox catalyst (eosin Y), and a chemically irreversible donor (triethanolamine). Side reactions with O2 do not significantly decrease the efficiency of photoredox catalysis. Energy transfer quenching between the electrode and the label can be lowered by increasing the distance between them. Washing‐free DNA detection had a detection limit of approximately 0.3 nm in buffer and 0.4 nm in serum without a washing step.
中文翻译:
表面等离子场诱导的光氧化还原催化和介导的电子转移,用于免清洗DNA检测。
依赖于距离的电磁辐射和电子转移通常分别用于免清洗的荧光和电化学生物测定中。在这项研究中,我们结合了两种与距离有关的现象,以进行灵敏的免洗DNA检测。距离相关的表面等离激元场引起表面结合的催化标记物的快速光氧化还原催化,而距离相关的介导的电子转移则允许电子从表面结合的标记物快速转移至电极。最佳系统由化学可逆的受体(Ru(NH 3)6 3+),化学可逆的光氧化还原催化剂(曙红Y)和化学不可逆的供体(三乙醇胺)组成。与O 2的副反应不会显着降低光氧化还原催化效率。电极和标签之间的能量转移猝灭可以通过增加它们之间的距离来降低。未经洗涤的DNA检测在不进行洗涤步骤的情况下,在缓冲液中的检测极限约为0.3 n m,在血清中的检测极限为0.4 n m。
更新日期:2020-06-17
中文翻译:
![](https://scdn.x-mol.com/jcss/images/paperTranslation.png)
表面等离子场诱导的光氧化还原催化和介导的电子转移,用于免清洗DNA检测。
依赖于距离的电磁辐射和电子转移通常分别用于免清洗的荧光和电化学生物测定中。在这项研究中,我们结合了两种与距离有关的现象,以进行灵敏的免洗DNA检测。距离相关的表面等离激元场引起表面结合的催化标记物的快速光氧化还原催化,而距离相关的介导的电子转移则允许电子从表面结合的标记物快速转移至电极。最佳系统由化学可逆的受体(Ru(NH 3)6 3+),化学可逆的光氧化还原催化剂(曙红Y)和化学不可逆的供体(三乙醇胺)组成。与O 2的副反应不会显着降低光氧化还原催化效率。电极和标签之间的能量转移猝灭可以通过增加它们之间的距离来降低。未经洗涤的DNA检测在不进行洗涤步骤的情况下,在缓冲液中的检测极限约为0.3 n m,在血清中的检测极限为0.4 n m。