Objective: The aim of this study is to investigate the pharmacokinetics of benzoic acid, 4- [[(2-hydroxyethyl)amino]carbonyl], methyl ester in rats after intravenous and oral administrations at doses of 3 mL/kg.

Methods: A rapid, high selective ultra-high performance liquid chromatographic electrospray quadrupole- time of flight mass spectrometry (UHPLC-ESI-Q-TOF/MS) method was applied to investigate the pharmacokinetics of benzoic acid, 4-[[(2-hydroxyethyl)amino]carbonyl]-, methyl ester with p-coumaric acid as internal standard (IS) in rats after intravenously and orally dosed.

Results: The pharmacokinetic data of benzoic acid, 4-[[(2-hydroxyethyl)amino]carbonyl]-, methyl ester was analyzed in the two-compartment open model. The main pharmacokinetic parameters were, respectively, 36.474 μg·h/mL, 12.59 μg·h/mL (AUC0→∞), and T1/2α was 0.14 h, 0.359 h; T1/2β was 3.046 h, 5.646 h after intravenous and oral administrations.

Conclusion: Benzoic acid, 4-[[(2-hydroxyethyl)amino] carbonyl]-, methyl ester was rapidly distributed in rat’s plasma with the absolute bioavailability of 34.5%.

目的：本研究的目的是研究静脉内和口服3 mL / kg剂量的苯甲酸，4-[[（（2-羟乙基）氨基]羰基]甲基酯在大鼠体内的药代动力学。

方法：采用快速，高选择性，超高效液相色谱电喷雾四极杆飞行时间质谱（UHPLC-ESI-Q-TOF / MS）方法研究苯甲酸4-[[（2-对-香豆酸作为内标（IS）的大鼠静脉内和口服给药后得到羟乙基）氨基]羰基]-甲酯。

结果：在两室开放模型中分析了苯甲酸4-[[（（2-羟乙基）氨基]羰基]-甲酯的药代动力学数据。主要药代动力学参数分别为36.474μg·h / mL，12.59μg·h / mL（AUC0→∞），T1 /2α为0.14 h，0.359 h。静脉内和口服给药后，T1 /2β为3.046小时，5.646小时。

结论：苯甲酸4-[[（（2-羟乙基）氨基]羰基]甲基酯在大鼠血浆中迅速分布，绝对生物利用度为34.5％。