DNA四面体模块作为多功能光学传感平台，可对miRNA，内切核酸酶和适体-配体复合物进行多重分析。,ACS Nano

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DNA四面体模块作为多功能光学传感平台，可对miRNA，内切核酸酶和适体-配体复合物进行多重分析。
ACS Nano ( IF 15.8 ) Pub Date : 2020-06-15 , DOI: 10.1021/acsnano.0c04031
Zhixin Zhou ₁ , Yang Sung Sohn ₂ , Rachel Nechushtai ₂ , Itamar Willner ₁

Affiliation

用于分析miRNA或内切核酸酶的传感模块由四面体组成，这些四面体以空间淬灭的配置和三个作为分析物识别元素的发夹单元功能化的三对不同的荧光猝灭剂对组成。三种不同的miRNA（miRNA-21，miRNA-221和miRNA-155）或三种不同的核酸内切酶（Nt.BbvCI，Eco RI和HindIII）解开相应的发夹，导致相应的荧光团的荧光开启并导致相应的分析物的多重检测。此外，引入了用于对适体配体复合物（配体= ATP，凝血酶，VEGF）进行多重分析的四面体模块。该模块包括用三个空间分离的荧光团-猝灭剂对修饰的边缘，这些荧光团-猝灭剂对被相应的适体链拉伸以产生开启的荧光状态。各个适体配体的形成将边缘重新配置为荧光团猝灭的笼状发夹结构，从而可以对适体-配体复合物进行多重分析。

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DNA Tetrahedra Modules as Versatile Optical Sensing Platforms for Multiplexed Analysis of miRNAs, Endonucleases, and Aptamer-Ligand Complexes.

The sensing modules for analyzing miRNAs or the endonucleases consist of tetrahedra functionalized with three different fluorophore–quencher pairs in spatially quenched configurations and hairpin units acting as recognition elements for the analytes. Three different miRNAs (miRNA-21, miRNA-221, and miRNA-155) or three different endonucleases (Nt.BbvCI, EcoRI, and HindIII) uncage the respective hairpins, leading to the switched-on fluorescence of the respective fluorophores and to the multiplex detection of the respective analytes. In addition, a tetrahedron module for the multiplexed analysis of aptamer ligand complexes (ligands = ATP, thrombin, VEGF) is introduced. The module includes edges modified with three spatially separated fluorophore–quencher pairs that were stretched by the respective aptamer strands to yield a switched-on fluorescent state. Formation of the respective aptamer ligands reconfigures the edges into fluorophore-quenched caged-hairpin structures that enable the multiplexed analysis of the aptamer–ligand complexes. The facile permeation of the tetrahedra structures into cells is used for the imaging of MCF-7 and HepG2 cancer cells and their discrimination from normal epithelial MCF-10A breast cells.

更新日期：2020-07-28

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