Mechanically Assisted Bioluminescence with Natural Luciferase.,Angewandte Chemie International Edition

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Mechanically Assisted Bioluminescence with Natural Luciferase.
Angewandte Chemie International Edition ( IF 16.1 ) Pub Date : 2020-06-15 , DOI: 10.1002/anie.202007440
Stefan Schramm ₁ , Marieh B Al-Handawi ₁ , Durga Prasad Karothu ₁ , Anastasiya Kurlevskaya ₁ , Patrick Commins ₁ , Yasuo Mitani ₂ , Chun Wu ₃ , Yoshihiro Ohmiya ₄ , Panče Naumov _{1,

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Affiliation

Mechanochemical analogues have recently been established for several enzymatic reactions, but they require periodic interruption of the reaction for sampling, dissolution, and (bio)chemical analysis to monitor their progress. By applying a mechanochemical procedure to induce bioluminescence analogous to that used by the marine ostracod Cypridina (Vargula) hilgendorfii, here we demonstrate that the light emitted by a bioluminescent reaction can be used to directly monitor the progress of a mechanoenzymatic reaction without sampling. Mechanical treatment of Cypridina luciferase with luciferin generates bright blue light which can be readily detected and analyzed spectroscopically. This mechanically assisted bioluminescence proceeds through a mechanism identical to that of bioluminescence in solution, but has higher activation energy due to being diffusion‐controlled in the viscous matrix. The results suggest that luciferases could be used as light‐emissive reporters of mechanoenzymatic reactions.

中文翻译：

机械辅助自然荧光素酶的生物发光。

近来已经建立了用于多种酶促反应的机械化学类似物，但是它们需要定期中断反应以进行采样，溶解和（生物）化学分析以监测其进展。通过应用机械化学过程来诱导类似于海洋原螯虾（Cargridina（Vargula）hilgendorfii）的生物发光，在这里我们证明了生物发光反应发出的光可用于直接监测机械酶促反应的进程而无需取样。用萤光素机械处理赛普里迪纳萤光素酶会产生明亮的蓝光，可以很容易地对其进行光谱检测和分析。这种机械辅助的生物发光通过与溶液中生物发光相同的机制进行，但由于在粘性基质中受扩散控制，因此具有更高的活化能。结果表明，荧光素酶可以用作机械酶反应的发光报告者。

更新日期：2020-06-15

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