线粒体外膜蛋白64的缺乏使水稻对水稻中的刺吸和咀嚼昆虫都有抗性。,Journal of Integrative Plant Biology

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线粒体外膜蛋白64的缺乏使水稻对水稻中的刺吸和咀嚼昆虫都有抗性。
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2020-06-15 , DOI: 10.1111/jipb.12983
Hui-Min Guo ₁ , Hai-Chao Li ₁ , Shi-Rong Zhou ₂ , Hong-Wei Xue ₂ , Xue-Xia Miao ₁

Affiliation

褐飞虱（BPH）和条纹茎bore（SSB）是水稻（Oryza sativa）产区中最具破坏性的害虫。筛选内源抗性基因是水稻抗虫育种的最实用策略。经过大规模田间BPH抗性表型筛选4年后，在水稻T-DNA插入种群（11,000个推定的纯合品系）中鉴定出45个对BPH具有高抗性的突变体。详细分析表明，水稻线粒体外膜蛋白64（OM64）缺乏。）基因导致对BPH的抗性增加。线粒体外膜蛋白64蛋白通过亚细胞定位而位于线粒体外膜中，其缺陷是组成型激活的过氧化氢（H ₂ O ₂）信号传导，从而刺激了抗菌性和对BPH的耐受性。所述om64突变体也显示出增强的抗SSB，咀嚼昆虫，这是由于促进茉莉酸的生物合成和相关应答。重要的是，om64植物在水稻产量相关性状上没有显着变化。这项研究证实了OM64通过调节H ₂ O _2的产生是水稻草食动物抗性的负调节剂。线粒体外膜蛋白64是通过基因缺失改善BPH和SSB抗性的潜在有效候选物。讨论了为什么om64突变体通过线粒体中的基因缺陷对刺吸昆虫和咀嚼昆虫具有抗性。

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Deficiency of mitochondrial outer membrane protein 64 confers rice resistance to both piercing-sucking and chewing insects in rice.

The brown planthopper (BPH) and striped stem borer (SSB) are the most devastating insect pests in rice (Oryza sativa) producing areas. Screening for endogenous resistant genes is the most practical strategy for rice insect‐resistance breeding. Forty‐five mutants showing high resistance against BPH were identified in a rice T‐DNA insertion population (11,000 putative homozygous lines) after 4 years of large‐scale field BPH‐resistance phenotype screening. Detailed analysis showed that deficiency of rice mitochondrial outer membrane protein 64 (OM64) gene resulted in increased resistance to BPH. Mitochondrial outer membrane protein 64 protein is located in the outer mitochondrial membrane by subcellular localization and its deficiency constitutively activated hydrogen peroxide (H₂O₂) signaling, which stimulated antibiosis and tolerance to BPH. The om64 mutant also showed enhanced resistance to SSB, a chewing insect, which was due to promotion of Jasmonic acid biosynthesis and related responses. Importantly, om64 plants presented no significant changes in rice yield‐related characters. This study confirmed OM64 as a negative regulator of rice herbivore resistance through regulating H₂O₂ production. Mitochondrial outer membrane protein 64 is a potentially efficient candidate to improve BPH and SSB resistance through gene deletion. Why the om64 mutant was resistant to both piercing‐sucking and chewing insects via a gene deficiency in mitochondria is discussed.

更新日期：2020-06-15

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