Phyllobilins from Senescence-Associated Chlorophyll Breakdown in the Leaves of Basil (Ocimum basilicum) Show Increased Abundance upon Herbivore Attack.,Journal of Agricultural and Food Chemistry

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Phyllobilins from Senescence-Associated Chlorophyll Breakdown in the Leaves of Basil (Ocimum basilicum) Show Increased Abundance upon Herbivore Attack.
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2020-06-10 , DOI: 10.1021/acs.jafc.0c02238
Simone Moser _{1,

2} , Theresia Erhart ₂ , Sigrid Neuhauser ₃ , Bernhard Kräutler ₂

Affiliation

In view of the common use of the herb basil (Ocimum basilicum) in nutrition and in phytomedicine, the contents of its leaves are of obvious interest. In extracts of fresh yellowish-green basil leaves, phyllobilins (PBs), which are bilin-type catabolites of chlorophyll (Chl), were detected using high-performance liquid chromatography (HPLC). Two such PBs, provisionally named Ob-nonfluorescent chlorophyll catabolite (NCC)-40 and Ob-YCC-45, exhibited previously unknown structures that were delineated by a thorough spectroscopic characterization. When basil leaves were infested with aphids or thrips or underwent fungal infections, areas with chlorosis were observed. HPLC analyses of the infested parts of leaves compared to those of the healthy parts showed a significant accumulation of PBs in the infested areas, demonstrating that the senescence-associated pheophorbide a oxygenase/phyllobilin (PAO/PB) pathway is activated by herbivore feeding and fungal infection.

中文翻译：

罗勒（Ocimum basilicum）叶片中与衰老相关的叶绿素分解产生的叶绿素在草食动物攻击后显示出增加的丰度。

考虑到罗勒（Ocimum basilicum）在营养和植物药中的普遍用途，其叶的含量引起了人们的明显兴趣。在新鲜的黄绿色罗勒叶提取物中，使用高效液相色谱（HPLC）检测叶绿素（PBs），叶绿素（PBs）是叶绿素（Chl）的Bilin型分解代谢产物。两个这样的PB，暂时命名为鄂-nonfluorescent叶绿素代谢产物（NCC）-40和鄂毕-YCC-45表现出以前未知的结构，通过彻底的光谱表征对其进行了描述。当罗勒叶被蚜虫或蓟马侵染或受到真菌感染时，会观察到有萎黄病的区域。与健康部位相比，叶片受侵染部位的HPLC分析表明，在受侵染区域中PB大量积累，表明草食动物摄食和真菌激活了衰老相关的脱镁叶绿酸加氧酶/叶胆素（PAO / PB）途径。感染。

更新日期：2020-07-08

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