Methyl brevifolincarboxylate (MBC) was isolated from ethyl acetate extract of Canarium album (Lour.) Raeusch. The structure was identified, and the effect on influenza A virus infection was evaluated. MBC exhibited inhibitory activity against influenza virus A/Puerto Rico/8/34 (H1N1) and A/Aichi/2/68 (H3N2) with IC₅₀ values of 27.16 ± 1.39 μM and 33.41 ± 2.34 μM. Mechanism studies indicated that MBC inhibited the replication of influenza A virus by targeting PB2 cap‐binding domain. Our results demonstrated MBC was a potent PB2 cap‐binding inhibitor and represented as a new type of promising lead compound for the development of anti‐influenza virus drugs from natural products.

中文翻译：

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Methyl brevifolincarboxylate，一种来自 Canarium Album (Lour.) Raeusch 的新型流感病毒 PB2 抑制剂。

从Canarium album (Lour.) Raeusch 的乙酸乙酯提取物中分离出短叶黄素羧酸甲酯 (MBC) 。确定了结构，并评估了对甲型流感病毒感染的影响。MBC 对流感病毒 A/Puerto Rico/8/34 (H1N1) 和 A/Aichi/2/68 (H3N2) 表现出抑制活性，IC ₅₀值为 27.16 ± 1.39 μM 和 33.41 ± 2.34 μM。机制研究表明，MBC 通过靶向 PB2 帽结合域来抑制甲型流感病毒的复制。我们的结果表明 MBC 是一种有效的 PB2 帽结合抑制剂，是一种新型的有前途的先导化合物，用于从天然产物中开发抗流感病毒药物。