2,6-DMBQ是一种新型的mTOR抑制剂，可在体外和体内降低胃癌的生长。,Journal of Experimental & Clinical Cancer Research

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2,6-DMBQ是一种新型的mTOR抑制剂，可在体外和体内降低胃癌的生长。
Journal of Experimental & Clinical Cancer Research ( IF 11.4 ) Pub Date : 2020-06-09 , DOI: 10.1186/s13046-020-01608-9
Xueyin Zu _{1,

2} , Xiaoli Ma _{1,

2} , Xiaomeng Xie _{1,

2} , Bingbing Lu _{1,

2} , Kyle Laster ₂ , Kangdong Liu _{1,

2,

3,

4} , Zigang Dong _{1,

2,

3,

4,

5} , Dong Joon Kim _{1,

2,

4}

Affiliation

据报道，发酵的小麦胚芽提取物在各种癌细胞中具有多种药理活性，包括抗氧化剂，抗细胞生长和细胞凋亡。尽管2,6-二甲氧基-1,4-苯醌（2,6-DMBQ）是苯醌化合物，并存在于发酵的小麦胚芽提取物中，但尚未研究其抗癌作用及其对胃癌的分子机制。通过MTT，软琼脂，细胞周期和膜联蛋白V分析确定2，6-DMBQ的抗癌作用。通过体外激酶测定和蛋白质印迹筛选潜在的候选蛋白。通过慢病毒感染shmTOR建立mTOR敲低细胞系。使用胃癌患者异种移植模型评估了2,6-DMBQ对肿瘤生长的影响。2，6-DMBQ显着降低胃癌细胞的细胞生长并诱导G1期细胞周期停滞和凋亡。2,6-DMBQ降低了mTOR的体外活性。2,6-DMBQ对细胞生长的抑制取决于mTOR蛋白的表达。值得注意的是，在体内小鼠模型中，2,6-DMBQ大大降低了患者衍生的异种移植胃肿瘤的生长。2,6-DMBQ是一种mTOR抑制剂，可用于治疗胃癌。它对胃癌患者具有治疗意义。6-DMBQ是一种mTOR抑制剂，可用于治疗胃癌。它对胃癌患者具有治疗意义。6-DMBQ是一种mTOR抑制剂，可用于治疗胃癌。它对胃癌患者具有治疗意义。

"点击查看英文标题和摘要"

2,6-DMBQ is a novel mTOR inhibitor that reduces gastric cancer growth in vitro and in vivo.

Fermented wheat germ extract has been reported to exert various pharmacological activities, including anti-oxidant, anti-cell growth and cell apoptosis in various cancer cells. Although 2,6-dimethoxy-1,4-benzoquinone (2,6-DMBQ) is a benzoquinone compound and found in fermented wheat germ extract, its anticancer effects and molecular mechanism(s) against gastric cancer have not been investigated. Anticancer effects of 2,6-DMBQ were determined by MTT, soft agar, cell cycle and Annexin V analysis. Potential candidate proteins were screened via in vitro kinase assay and Western blotting. mTOR knockdown cell lines were established by lentiviral infection with shmTOR. The effect of 2,6-DMBQ on tumor growth was assessed using gastric cancer patient-derived xenograft models. 2,6-DMBQ significantly reduced cell growth and induced G1 phase cell cycle arrest and apoptosis in gastric cancer cells. 2,6-DMBQ reduced the activity of mTOR in vitro. The inhibition of cell growth by 2,6-DMBQ is dependent upon the expression of the mTOR protein. Remarkably, 2,6-DMBQ strongly reduced patient-derived xenograft gastric tumor growth in an in vivo mouse model. 2,6-DMBQ is an mTOR inhibitor that can be useful for treating gastric cancer. It has therapeutic implications for gastric cancer patients.

更新日期：2020-06-09

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